Böhmler Judith, Haidara Hamidou, Ponche Arnaud, Ploux Lydie
Institut de Science des Matériaux de Mulhouse, CNRS-UMR7361, University of Strasbourg/University of Haute-Alsace, UMR7361, Mulhouse, France.
ACS Biomater Sci Eng. 2015 Aug 10;1(8):693-704. doi: 10.1021/acsbiomaterials.5b00151. Epub 2015 Jun 25.
An essential yet never addressed parameter for the control of bacteria on functionalized biomaterial is surely the accessibility and heterogeneity of the functional groups immobilized on the surface. In this context, we investigated the colonization ( K12, RP62A) of precisely engineered surfaces revealing various densities of NH and CH functional groups. We demonstrated for the first time nonlinear relationships between the NH/CH surface fraction and the quantity of adhered, adhering or detaching bacteria. Plateaus and transition zones were related to the range of NH/CH surface fraction offering stability or sharp variation in bacterium/surface interactions. The nonlinear behavior was attributed to the discrete distribution of positive charges revealed by the bacterial membrane in the continuum of negative charges resulting from the phospholipids, which may correlate with one single specific distribution of positive NH charges on the material surface, because of electrostatic, repulsive interactions occurring at the local, molecular scale.
对于功能化生物材料上细菌的控制，一个至关重要却从未被提及的参数无疑是固定在表面的官能团的可及性和异质性。在此背景下，我们研究了精确设计的表面（具有各种密度的NH和CH官能团）上的细菌定殖（K12、RP62A）情况。我们首次证明了NH/CH表面分数与附着、正在附着或分离的细菌数量之间存在非线性关系。平台区和过渡区与NH/CH表面分数的范围有关，该范围提供了细菌/表面相互作用的稳定性或急剧变化。这种非线性行为归因于细菌膜在磷脂产生的连续负电荷中所揭示的正电荷的离散分布，由于在局部分子尺度上发生的静电排斥相互作用，这可能与材料表面正NH电荷的单一特定分布相关。
