Subendran Satishkumar, Kang Chun-Wei, Chen Chia-Yuan
Department of Mechanical Engineering, National Cheng Kung University, No. 1 University Road, Tainan 701, Taiwan.
Micromachines (Basel). 2021 Jan 9;12(1):68. doi: 10.3390/mi12010068.
The zebrafish is acknowledged as a reliable species of choices for biomechanical-related investigations. The definite quantification of the hydrodynamic flow physics caused by behavioral patterns, particularly in the zebrafish tail beat, is critical for a comprehensive understanding of food toxicity in this species, and it can be further interpreted for possible human responses. The zebrafish's body size and swimming speed place it in the intermediate flow regime, where both viscous and inertial forces play significant roles in the fluid-structure interaction. This pilot work highlighted the design and development of a novel microfluidic device coupled with a shape memory alloy (SMA) actuator to immobilize the zebrafish within the observation region for hydrodynamic quantification of the tail-beating behavioral responses, which may be induced by the overdose of food additive exposure. This study significantly examined behavioral patterns of the zebrafish in early developmental stages, which, in turn, generated vortex circulation. The presented findings on the behavioral responses of the zebrafish through the hydrodynamic analysis provided a golden protocol to assess the zebrafish as an animal model for new drug discovery and development.
斑马鱼被认为是生物力学相关研究的可靠选择物种。由行为模式引起的流体动力学流动物理特性的明确量化，特别是斑马鱼尾鳍摆动方面的量化，对于全面了解该物种的食物毒性至关重要，并且可以进一步解读其对人类可能产生的反应。斑马鱼的体型和游泳速度使其处于中间流动状态，在这种状态下粘性力和惯性力在流固相互作用中都起着重要作用。这项初步工作重点介绍了一种新型微流控装置的设计与开发，该装置与形状记忆合金（SMA）致动器相结合，用于将斑马鱼固定在观察区域内，以便对可能由过量接触食品添加剂引起的尾鳍摆动行为反应进行流体动力学量化。本研究对斑马鱼早期发育阶段的行为模式进行了显著研究，这些行为模式反过来又产生了涡旋环流。通过流体动力学分析得出的关于斑马鱼行为反应的研究结果，为评估斑马鱼作为新药发现和开发的动物模型提供了一个黄金方案。
