Mertes Verena, Bekkelund Alexander Kashulin, Lagos Leidy, Ciani Elia, Colquhoun Duncan, Haslene-Hox Hanne, Sletta Håvard, Sørum Henning, Winther-Larsen Hanne Cecilie
Section of Pharmacology and Pharmaceutical Biosciences and Centre of Integrative Microbial Evolution, Department of Pharmacy, University of Oslo, 0315 Oslo, Norway.
Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, 1430 Ås, Norway.
Vaccines (Basel). 2021 Jan 9;9(1):34. doi: 10.3390/vaccines9010034.
Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens ssp. and . The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we tested isolated membrane vesicles (MVs) as possible vaccine candidates based on previous trials in zebrafish () indicating promising vaccine efficacy. Here, the MV vaccine-candidates were tested in their natural hosts, Atlantic cod ( L.) and Nile tilapia (). Injection of MVs did not display any toxicity or other negative influence on the fish and gene expression analysis indicated an influence on the host immune response. However, unlike in other tested fish species, a protective immunity following vaccine application and immunization period could not be detected in the Atlantic cod or tilapia. Further in vivo studies are required to achieve a better understanding of the development of immunological memory in different fish species.
鱼类弗朗西斯菌病由兼性胞内革兰氏阴性细菌病原体亚种和引起。该疾病正在影响全球范围内的养殖鱼类和野生鱼类，目前尚无商业疫苗可用。在本研究中，基于先前在斑马鱼（）中的试验表明有前景的疫苗效力，我们测试了分离的膜泡（MVs）作为可能的疫苗候选物。在此，MV疫苗候选物在其自然宿主大西洋鳕鱼（L.）和尼罗罗非鱼（）中进行了测试。注射MVs对鱼未显示出任何毒性或其他负面影响，基因表达分析表明对宿主免疫反应有影响。然而，与其他测试鱼类不同，在大西洋鳕鱼或罗非鱼中未检测到疫苗应用和免疫期后的保护性免疫。需要进一步的体内研究以更好地了解不同鱼类中免疫记忆的发展。
