Institute of Microbiology, Department of Microbial Proteomics, Center for Functional Genomics of Microbes, University of Greifswald, 17489, Greifswald, Germany.
Imaging Center of the Department of Biology, University of Greifswald, 17489, Greifswald, Germany.
Sci Rep. 2022 Feb 22;12(1):3003. doi: 10.1038/s41598-022-06130-w.
Bacterial kidney disease (BKD) is a chronic bacterial disease affecting both wild and farmed salmonids. The causative agent for BKD is the Gram-positive fish pathogen Renibacterium salmoninarum. As treatment and prevention of BKD have proven to be difficult, it is important to know and identify the key bacterial proteins that interact with the host. We used subcellular fractionation to report semi-quantitative data for the cytosolic, membrane, extracellular, and membrane vesicle (MV) proteome of R. salmoninarum. These data can aid as a backbone for more targeted experiments regarding the development of new drugs for the treatment of BKD. Further analysis was focused on the MV proteome, where both major immunosuppressive proteins P57/Msa and P22 and proteins involved in bacterial adhesion were found in high abundance. Interestingly, the P22 protein was relatively enriched only in the extracellular and MV fraction, implicating that MVs may play a role in host-pathogen interaction. Compared to the other subcellular fractions, the MVs were also relatively enriched in lipoproteins and all four cell wall hydrolases belonging to the New Lipoprotein C/Protein of 60 kDa (NlpC/P60) family were detected, suggesting an involvement in the formation of the MVs.
细菌性肾病（BKD）是一种影响野生和养殖鲑鱼的慢性细菌性疾病。BKD 的病原体是革兰氏阳性鱼类病原体鲑鱼鳜鱼。由于 BKD 的治疗和预防已被证明很困难，因此了解和识别与宿主相互作用的关键细菌蛋白非常重要。我们使用亚细胞分级分离法报告了鲑鱼鳜鱼的细胞质、膜、细胞外和膜囊泡（MV）蛋白质组的半定量数据。这些数据可以作为针对 BKD 治疗新药物开发的更有针对性的实验的基础。进一步的分析集中在 MV 蛋白质组上，其中发现了大量主要的免疫抑制蛋白 P57/Msa 和 P22 以及参与细菌黏附的蛋白。有趣的是，P22 蛋白仅在细胞外和 MV 部分相对丰富，这表明 MV 可能在宿主-病原体相互作用中发挥作用。与其他亚细胞部分相比，MV 也相对富含脂蛋白，并且检测到属于新型脂蛋白 C/60 kDa 蛋白（NlpC/P60）家族的所有四个细胞壁水解酶，表明其参与了 MV 的形成。
