Jurado Rocío, Gálvez Natividad
Department of Inorganic Chemistry, University of Granada, 18071 Granada, Spain.
Nanomaterials (Basel). 2021 Jan 8;11(1):146. doi: 10.3390/nano11010146.
The coupling of proteins that can assemble, recognise or mineralise specific inorganic species is a promising strategy for the synthesis of nanoscale materials with a controllable morphology and functionality. Herein, we report that apoferritin protein amyloid fibrils (APO) have the ability to assemble and/or synthesise various metal and metal compound nanoparticles (NPs). As such, we prepared metal NP-protein hybrid bioconjugates with improved optical and magnetic properties by coupling diverse gold (AuNPs) and magnetic iron oxide nanoparticles (MNPs) to apoferritin amyloid fibrils and compared them to the well-known β-lactoglobulin (BLG) protein. In a second approach, we used of solvent-exposed metal-binding residues in APO amyloid fibrils as nanoreactors for the in situ synthesis of gold, silver (AgNPs) and palladium nanoparticles (PdNPs). Our results demonstrate, the versatile nature of the APO biotemplate and its high potential for preparing functional hybrid bionanomaterials. Specifically, the use of apoferritin fibrils as vectors to integrate magnetic MNPs or AuNPs is a promising synthetic strategy for the preparation of specific contrast agents for early in vivo detection using various bioimaging techniques.
能够组装、识别或矿化特定无机物种的蛋白质偶联，是合成具有可控形态和功能的纳米级材料的一种有前景的策略。在此，我们报道脱铁铁蛋白蛋白质淀粉样纤维（APO）具有组装和/或合成各种金属及金属化合物纳米颗粒（NP）的能力。因此，我们通过将不同的金纳米颗粒（AuNP）和磁性氧化铁纳米颗粒（MNP）偶联到脱铁铁蛋白淀粉样纤维上，制备了具有改善的光学和磁性特性的金属NP-蛋白质杂化生物共轭物，并将它们与著名的β-乳球蛋白（BLG）蛋白质进行比较。在第二种方法中，我们将APO淀粉样纤维中暴露于溶剂的金属结合残基用作原位合成金、银（AgNP）和钯纳米颗粒（PdNP）的纳米反应器。我们的结果证明了APO生物模板的多功能性质及其在制备功能性杂化生物纳米材料方面的巨大潜力。具体而言，使用脱铁铁蛋白纤维作为载体整合磁性MNP或AuNP，是一种有前景的合成策略，可用于制备使用各种生物成像技术进行早期体内检测的特定造影剂。
