Department of Burn and Plastic Surgery, The Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, China.
Burn Institute of PLA, Department of Burn Surgery, The First Affiliated Hospital. Naval Medical University, Shanghai, China.
J Int Med Res. 2021 Jan;49(1):300060520984652. doi: 10.1177/0300060520984652.
The role of tumor necrosis factor (TNF)-α small interfering (si)RNA alveolar epithelial cell (AEC)-targeting nanoparticles in lung injury is unclear.
Sixty C57BL/6J mice with sepsis were divided into normal, control, sham, 25 mg/kg, 50 mg/kg, and 100 mg/kg siRNA AEC-targeting nanoparticles groups (n = 10 per group). The wet:dry lung weight ratio, and hematoxylin and eosin staining, western blotting, and enzyme-linked immunosorbent assays for inflammatory factors were conducted to compare differences among groups.
The wet:dry ratio was significantly lower in control and sham groups than other groups. TNF-α siRNA AEC-targeting nanoparticles significantly reduced the number of eosinophils, with significantly lower numbers in the 50 mg/kg group than in 25 mg/kg and 100 mg/kg groups. The nanoparticles also significantly reduced the expression of TNF-α, B-cell lymphoma-2, caspase 3, interleukin (IL)-1β, and IL-6, with TNF-α expression being significantly lower in the 50 mg/kg group than in 25 mg/kg and 100 mg/kg groups.
TNF-α siRNA AEC-targeting nanoparticles appear to be effective at improving lung injury-related sepsis, and 50 mg/kg may be a preferred dose option for administration.
肿瘤坏死因子 (TNF)-α 小干扰 (si)RNA 肺泡上皮细胞 (AEC)靶向纳米颗粒在肺损伤中的作用尚不清楚。
将 60 只脓毒症 C57BL/6J 小鼠分为正常组、对照组、假手术组、25mg/kg、50mg/kg 和 100mg/kg siRNA AEC 靶向纳米颗粒组（每组 10 只）。比较各组间的肺湿重/干重比、苏木精-伊红染色、Western blot 和酶联免疫吸附试验检测炎症因子的差异。
对照组和假手术组的湿重/干重比明显低于其他组。TNF-α siRNA AEC 靶向纳米颗粒明显减少嗜酸性粒细胞数量，50mg/kg 组比 25mg/kg 和 100mg/kg 组明显减少。纳米颗粒还明显降低 TNF-α、B 细胞淋巴瘤-2、半胱氨酸天冬氨酸蛋白酶 3、白细胞介素 (IL)-1β 和 IL-6 的表达，50mg/kg 组 TNF-α 的表达明显低于 25mg/kg 和 100mg/kg 组。
TNF-α siRNA AEC 靶向纳米颗粒似乎能有效改善与脓毒症相关的肺损伤，50mg/kg 可能是更优的给药剂量选择。
