Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
Pharmacognosy and Phytochemistry Laboratory, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
J Toxicol Environ Health A. 2021 Apr 18;84(8):345-355. doi: 10.1080/15287394.2020.1871144. Epub 2021 Jan 12.
is a weed thought to have originated in Brazil, where it is present in abundant quantities, but also this plant is also found in south-central Florida, Indian Ocean Islands, and the Pacific Islands. produces neurotoxicity that adversely affects livestock breeding with heavy animal losses and consequent negative impact on Brazil's economy. The aim of this study was to determine the chemical profile, cytotoxic and genotoxic effects of ethanolic extracts of collected in winter (leaf extract) and summer (leaf extract and leaf + flower extract) using an model of human neuroblastoma cell line SH-SY5Y. Phytochemical screening demonstrated the presence of alkaloids, flavonoids, and apolar compounds. Rutin, quercetin, and swainsonine were detected by HPLC and GC/MS, respectively. Phosphorus, potassium, iron, and zinc were the inorganic elements found. Extracts produced cytotoxicity at all concentrations tested (7-4,000 μg/ml) as evidenced by the colorimetric assay [3-(4,5-dimethyl-thiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT)]. Based upon the alkaline comet assay extracts were found to induce genotoxicity at concentrations ranging from 0.437 to 7 μg/ml. DNA damage produced by extracts was affirmed using a modified comet assay with the enzymes Endo III and FPG in a concentration dependent manner. Further, enzyme-modified comet assay showed both oxidized purines and pyrimidines, and consequently oxidative stress was related to genomic instability and cell death. Data suggest that low concentrations of ethanolic extracts of (different seasons) induced increased DNA damage related to oxidative stress and chemical composition.
是一种杂草，被认为起源于巴西，在那里它大量存在，但这种植物也存在于佛罗里达州中南部、印度洋岛屿和太平洋岛屿。 产生神经毒性，对畜牧业养殖造成不利影响，导致牲畜大量死亡，对巴西经济造成负面影响。本研究旨在使用人神经母细胞瘤细胞系 SH-SY5Y 模型，确定冬季（叶提取物）和夏季（叶提取物和叶+花提取物）采集的 的化学特征、细胞毒性和遗传毒性作用。植物化学筛选表明存在生物碱、类黄酮和非极性化合物。通过 HPLC 和 GC/MS 分别检测到芦丁、槲皮素和 swainsonine。发现无机元素有磷、钾、铁和锌。提取物在所有测试浓度（7-4,000μg/ml）下均产生细胞毒性，这可通过比色法 [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴盐（MTT）] 证明。根据碱性彗星试验，提取物在 0.437 至 7μg/ml 的浓度范围内诱导遗传毒性。提取物产生的 DNA 损伤通过用酶 Endo III 和 FPG 进行的改良彗星试验得到证实，呈浓度依赖性。此外，酶修饰的彗星试验表明氧化嘌呤和嘧啶，因此氧化应激与基因组不稳定性和细胞死亡有关。数据表明，不同季节的 乙醇提取物的低浓度诱导与氧化应激和化学成分有关的 DNA 损伤增加。
