Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Xinghua Industrial Research Centre for Food Science and Human Health, China Agricultural University, Xinghua, 225700 Jiangsu, China.
Carbohydr Polym. 2021 Mar 1;255:117379. doi: 10.1016/j.carbpol.2020.117379. Epub 2020 Nov 10.
To meet the increasing demand for polysaccharide-stabilized selenium nanoparticles (PS-Se°NPs) for industrial applications, in this study, we employed three commonly-used polysaccharides (i.e. chitosan (CS), carrageenan (Cg) and Gum Arabic (GA)) to fabricate Se°NPs, and compared their functional properties and their physicochemical stabilities, under varying conditions (ionic strength, pH, and temperature). The results showed that CS-Se°NPs had higher storage stability but easily aggregated in 500 mM NaCl or at pH > 8 because of their surface cationic groups. This, however, improved their DPPH free radical scavenging and antitumor activities. Compared to CS-Se°NPs, the anionic groups in Cg-Se°NPs increased their resistant to pH variations and reduced cellular toxicity, and the multi-branched structure of GA-Se°NPs increased their thermal stability. Overall, this study shows that the behaviors of PS-Se°NPs depends mainly on ionizable functional groups and the structure of polysaccharides, contributing to the development of PS-Se°NPs with applicability in the food and pharmaceutical industries.
为满足多糖稳定硒纳米颗粒（PS-Se°NPs）在工业应用中的日益增长的需求，在本研究中，我们采用了三种常用的多糖（壳聚糖（CS）、卡拉胶（Cg）和阿拉伯胶（GA））来制备 Se°NPs，并比较了它们在不同条件（离子强度、pH 值和温度）下的功能特性和物理化学稳定性。结果表明，由于表面带正电荷基团，CS-Se°NPs 具有更高的储存稳定性，但在 500mM NaCl 或 pH 值>8 时容易聚集。然而，这提高了它们的 DPPH 自由基清除和抗肿瘤活性。与 CS-Se°NPs 相比，Cg-Se°NPs 中的阴离子基团增加了其对 pH 变化的抵抗力和降低了细胞毒性，GA-Se°NPs 的多分支结构增加了其热稳定性。总的来说，本研究表明 PS-Se°NPs 的行为主要取决于可离子化的功能基团和多糖的结构，这有助于开发在食品和制药工业中具有适用性的 PS-Se°NPs。
