Xu Xindong, Pan Yuxue, Liu Xiaoying, Han Zhong, Chen Shan
College of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.
Foods. 2023 Jan 27;12(3):563. doi: 10.3390/foods12030563.
Selenium nanoparticles (SeNPs) are among the emerging selenium supplements because of their high bioactivity and low toxicity. However, bare SeNPs are prone to activity loss caused by aggregation and sedimentation. This study aims to stabilize SeNPs with curdlan (CUR), a polysaccharide, to maintain or even enhance their biological activity. Herein, the stable SeNPs were constructed via the unique conformational transition of CUR induced by alkali-neutralization (AN) pretreatment. The physicochemical properties and structures of the prepared SeNPs were characterized by dynamic light scattering (DLS), UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and free-radical-scavenging activity assays. The results show that most SeNPs are stabilized within the triple helix of CUR that has been pretreated with high-intensity AN treatment. These amorphous, small-sized (average size was 53.6 ± 17.7 nm), and stabilized SeNPs have significantly enhanced free-radical-scavenging ability compared to the control and can be well-stabilized for at least 240 days at 4 °C. This work indicates that CUR, as a food additive, can be used to well-stabilize SeNPs by AN pretreatment and provides a facile method to prepare and enhance the stability and bioactivity of SeNPs via triple-helix conformational transition.
由于具有高生物活性和低毒性，硒纳米颗粒（SeNPs）属于新兴的硒补充剂。然而，裸露的SeNPs容易因聚集和沉淀而导致活性丧失。本研究旨在用多糖凝胶多糖（CUR）稳定SeNPs，以维持甚至增强其生物活性。在此，通过碱中和（AN）预处理诱导的CUR独特构象转变构建了稳定的SeNPs。通过动态光散射（DLS）、紫外可见光谱、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、透射电子显微镜（TEM）和自由基清除活性测定对制备的SeNPs的物理化学性质和结构进行了表征。结果表明，大多数SeNPs稳定在经过高强度AN处理预处理的CUR三螺旋结构内。这些无定形、小尺寸（平均尺寸为53.6±17.7nm）且稳定的SeNPs与对照相比具有显著增强的自由基清除能力，并且在4℃下可良好稳定至少240天。这项工作表明，CUR作为一种食品添加剂，可通过AN预处理很好地稳定SeNPs，并提供了一种通过三螺旋构象转变制备和增强SeNPs稳定性及生物活性的简便方法。
