玉竹低聚糖：结构表征及其对 LPS 诱导的腹膜炎的治疗作用。
Oligosaccharides from Polygonatum Cyrtonema Hua: Structural characterization and treatment of LPS-induced peritonitis in mice.
机构信息
National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China; Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
出版信息
Carbohydr Polym. 2021 Mar 1;255:117392. doi: 10.1016/j.carbpol.2020.117392. Epub 2020 Nov 10.
Fructooligosaccharide was isolated from Polygonatum Cyrtonema Hua (PFOS) for the first time. Structure characterized using FT-IR, MALDI-TOF-MS, NMR, AFM, and TEM, indicated that PFOS was graminan-type fructan with a degree of polymerization ranging from 5 to 10. A murine model of lipopolysaccharide (LPS)-induced peritonitis was used to evaluate the in vivo anti-inflammatory and lung protective efficacy of PFOS. The result shown that pretreatment with PFOS (1.0 mg/mL) in peritonitis-induced mice could significantly inhibit the level of pro-inflammatory cytokines (TNF-α, IL-1β) in serum (P < 0.001), increase mice survival rate from 12.5 % to 54 % (P < 0.05), and alleviated lung injury through ameliorating the damage of the pulmonary cellular architecture and reducing inflammatory monocyte accumulation in lung tissue. This effect of oligosaccharides could explain the traditional usage of P. cyrtonema as a tonic medicine for respiratory problems and it could be used as a potential natural ingredient with anti-inflammatory activity.
首次从黄精（Polygonatum Cyrtonema Hua）中分离得到低聚果糖（PFOS）。使用 FT-IR、MALDI-TOF-MS、NMR、AFM 和 TEM 对其结构进行了表征，结果表明 PFOS 是一种支链型果聚糖，聚合度为 5-10。采用脂多糖（LPS）诱导的腹膜炎小鼠模型评价 PFOS 的体内抗炎和肺保护作用。结果表明，PFOS（1.0 mg/mL）预处理可显著抑制腹膜炎诱导的小鼠血清中促炎细胞因子（TNF-α、IL-1β）水平（P < 0.001），将小鼠的存活率从 12.5%提高到 54%（P < 0.05），并通过改善肺细胞结构损伤和减少肺组织中炎症性单核细胞聚集来减轻肺损伤。这种低聚糖的作用可以解释黄精作为一种治疗呼吸系统问题的滋补药物的传统用途，它可以作为一种具有抗炎活性的潜在天然成分。
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