Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt.
Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt; Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, MO, United States.
Carbohydr Polym. 2021 Mar 1;255:117484. doi: 10.1016/j.carbpol.2020.117484. Epub 2020 Dec 5.
Wound dressing composed of chitosan, based crosslinked gelatin/ polyvinyl pyrrolidone, embedded silver nanoparticles were fabricated using solution casting method. The membrane was characterized by FTIR, SEM and TGA. Glutaraldehyde (0.5 %) was used for the crosslinking of membrane components and associated with 7-folds boosted mechanical performance, 28 % more hydrolytic stability, 3-folds thickness reduction and morphological roughness. Silver nanoparticles were characterized by UV-vis, XRD and TEM for an average size of 9.9 nm. The membrane with higher concentration of silver nanoparticles showed maximum antibacterial activity against human pathogenic bacteria; and the measured inhibition zones ranged from 1.5 to 3 cm. The activity of the particles ranged from severe to complete reduction in Penicillin, Erythromycin and Macrolide family's resistance genes expression such as β-Lactamase, mecA and erm. This developed membrane can serve as promising and cost-effective system against severe diabetic and burn wound infections.
采用溶液浇铸法制备了壳聚糖为基础的交联明胶/聚乙烯吡咯烷酮载银纳米粒子伤口敷料。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和热重分析（TGA）对膜进行了表征。戊二醛（0.5%）用于膜成分的交联，并与 7 倍的机械性能提高、28%的水解稳定性提高、3 倍的厚度减少和形态粗糙度相关联。通过紫外-可见分光光度法（UV-vis）、X 射线衍射（XRD）和透射电子显微镜（TEM）对银纳米粒子进行了表征，平均粒径为 9.9nm。含较高浓度银纳米粒子的膜对人体致病菌表现出最大的抗菌活性；测量的抑菌圈范围为 1.5 至 3 厘米。这些粒子的活性导致青霉素、红霉素和大环内酯类抗生素家族的耐药基因表达如β-内酰胺酶、mecA 和 erm 严重至完全减少。这种开发的膜可以作为一种有前途且具有成本效益的系统，用于严重的糖尿病和烧伤伤口感染。
