State Key Laboratory of Food Science and Technology, China-Canada Joint Lab. of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi Province, 330047, China.
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab. of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi Province, 330047, China.
Carbohydr Polym. 2021 Mar 1;255:117502. doi: 10.1016/j.carbpol.2020.117502. Epub 2020 Dec 13.
Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated. In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis. Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied. JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain. JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues. Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1. This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.
柱状田头菇是一种常见的活性多糖来源，但它们的精细结构尚不清楚。本研究从柱状田头菇（JACP）的碱性提取物中分离得到四个多糖组分，并通过 HPAEC-PAD、甲基化结合 GC-MS 和 1D/2D NMR 分析比较了它们的化学组成和结构。结果表明，四个组分的理化性质，包括单糖组成、分子量、黏度和表面形貌均有较大差异。JACP-30 被鉴定为一种以α-(1→6)-半乳糖吡喃基作为主链的岩藻糖葡甘露聚糖。JACP-50p 和 JACP-80r 被表征为β-(1→6)-葡聚糖，其侧链由末端和 3-取代的β-吡喃葡萄糖基残基组成，每隔三个残基连接在 O-3 上。同样，JACP-80 的主链由β-(1→6)-连接的葡萄糖基和β-(1→3,6)-连接的葡萄糖基残基组成，比例为 4:1。这项工作为理解柱状田头菇多糖提供了更多信息，进一步指导其生物学研究，并开发其在食品和生物医药行业的应用。
