College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Department of Pediatrics, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
Carbohydr Polym. 2020 May 15;236:116056. doi: 10.1016/j.carbpol.2020.116056. Epub 2020 Feb 22.
The aim of the present study is to characterize the structure of a novel natural polysaccharide from Agrocybe aegirita (AAPS) and evaluate its anti-aging activity. The MALLS and GC-MS analysis indicated that the AAPS with molecular weights of 1.81 × 10 Da was mainly composed by rhamnose, fucose, mannose, and glucose in a molar ratio of 2.90:10.25:3.70:38.27. The FT-IR and NMR analysis showed that the backbone of AAPS was α-L-Rhap-(1→6)-β-D-Glcp-(1→2)-α-L-Fucp-(1→6)-α-D-Glcp-(1→5)-α-L-Araf-(1→4)-β-D-GlcpA-(1→5)-α-L-Araf-(1→6)-α-D-Manp-(1→6)-α-D-Manp-(1→2)-α-L-Fucp-(1→6)-β-D-Glap-(1→2)-α-L-Rhap-(1→6)-β-D-Galp-(1→, which linked with two side chains α-L-Fucp-(1→6)-β-D-Glcp-(1→6)-β-D-Manp-(1→ and α-D-Xylp-(1→2)-α-L-Fucp-(1→5) -α-D-Araf-(1→6)-β-D-Galp-(1→ at O at H-4-arabinose and the terminal Galp residues, respectively. The MRC-5 cells induced by HO were used to explore the anti-ageing effect and its underlying mechanism of AAPS. It showed a potent anti-ageing activity, representing by the increased cell viability and β-Gal viability, prevented G1-phase cell-cycle arrest, and decreased mitochondrial membrane potential. The polysaccharides extracted from A. aegirita might be applied in functional food as anti-ageing ingredient.
本研究旨在对一种新型天然多糖（AAPS）的结构进行表征，并评估其抗衰老活性。MALLS 和 GC-MS 分析表明，分子量为 1.81×10 Da 的 AAPS 主要由鼠李糖、岩藻糖、甘露糖和葡萄糖组成，摩尔比为 2.90:10.25:3.70:38.27。FT-IR 和 NMR 分析表明，AAPS 的骨架是由 α-L-Rhap-(1→6)-β-D-Glcp-(1→2)-α-L-Fucp-(1→6)-α-D-Glcp-(1→5)-α-L-Araf-(1→4)-β-D-GlcpA-(1→5)-α-L-Araf-(1→6)-α-D-Manp-(1→6)-α-D-Manp-(1→2)-α-L-Fucp-(1→6)-β-D-Glap-(1→2)-α-L-Rhap-(1→6)-β-D-Galp-(1→组成的，该骨架通过两条侧链α-L-Fucp-(1→6)-β-D-Glcp-(1→6)-β-D-Manp-(1→和α-D-Xylp-(1→2)-α-L-Fucp-(1→5)-α-D-Araf-(1→6)-β-D-Galp-(1→连接到 H-4-阿拉伯糖和末端 Galp 残基上。用 HO 诱导的 MRC-5 细胞用于探索 AAPS 的抗衰老作用及其潜在机制。结果表明，AAPS 具有很强的抗衰老活性，表现为细胞活力和β-Gal 活力增加，阻止 G1 期细胞周期停滞，并降低线粒体膜电位。从 A. aegirita 中提取的多糖可能作为抗衰老成分应用于功能性食品。
