State Key Laboratory of Food Science and Resources, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi Province 330047, China.
State Key Laboratory of Food Science and Resources, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi Province 330047, China; Food Laboratory of Zhongyuan, Luohe 462300, Henan, China.
Carbohydr Polym. 2024 Sep 1;339:122235. doi: 10.1016/j.carbpol.2024.122235. Epub 2024 May 9.
This study explored the physicochemical properties and structural characteristics of Agrocybe cylindracea polysaccharides at four developmental stages, as well as their dynamic evolution during maturation. Results showed that the polysaccharides from A. cylindracea water extract exhibited similar structural characteristics across all four maturity stages, despite a significant reduction in yields. Four water-soluble heteroglycans, including one high molecular weight (ACPM-Et50-I) and three low molecular weight (ACPM-Et50-II, ACPM-Et60, ACPM-Et80), were isolated from A. cylindracea at each maturity stage. ACPM-Et50-I was identified as branched heterogalactans, while ACPM-Et60 and ACPM-Et80 were branched heteroglucans. However, ACPM-Et50-II was characterized as a branched glucuronofucogalactoglucan at the tide-turning stage but a glucuronofucoglucogalactan at the pileus expansion stage due to the increase of its α-(1 → 6)-D-Galp. In general, although the structural skeletons of most A. cylindracea heteroglycans were similar during maturation as shown by their highly consistent glycosyl linkages, there were still differences in the distribution of some heteroglucans. This work has for the first time reported a glucuronofucogalactoglucan in A. cylindracea and its dynamic evolution during maturation, which may facilitate the potential application of A. cylindracea in food and biomedicine industries.
本研究探讨了四个发育阶段的柱状田头菇多糖的物理化学性质和结构特征，以及它们在成熟过程中的动态演变。结果表明，尽管产量显著降低，但柱状田头菇水提物的多糖在所有四个成熟阶段都表现出相似的结构特征。从各个成熟阶段的柱状田头菇中分离得到了四种水溶性杂多糖，包括一种高分子量（ACPM-Et50-I）和三种低分子量（ACPM-Et50-II、ACPM-Et60、ACPM-Et80）。ACPM-Et50-I 被鉴定为支化杂半乳糖聚糖，而 ACPM-Et60 和 ACPM-Et80 则是支化杂葡聚糖。然而，ACPM-Et50-II 在转折期表现为支化葡糖醛酸岩藻半乳糖葡聚糖，但在菌盖扩张期则表现为葡糖醛酸岩藻葡聚糖，这是由于其α-(1→6)-D-Galp 的增加。总的来说，尽管大多数柱状田头菇杂多糖在成熟过程中的结构骨架相似，表现为高度一致的糖苷键，但一些杂葡聚糖的分布仍存在差异。本研究首次报道了柱状田头菇中的一种葡糖醛酸岩藻半乳糖葡聚糖及其在成熟过程中的动态演变，这可能有助于柱状田头菇在食品和生物医学工业中的潜在应用。
