Department of Microbiology, Molecular Microbiology, Oslo University Hospital, P.O. Box 4950, 0424, Oslo, Norway.
Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, Trondheim, Norway.
Sci Rep. 2021 Jan 12;11(1):474. doi: 10.1038/s41598-020-80043-4.
Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10-20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell.
拓扑异构酶 IV（TopoIV）是一种重要的细菌酶，可解开新复制的 DNA 并促进子染色体的分离。在细菌中，DNA 复制和分离是同时进行的过程。这意味着 TopoIV 必须在复制过程中不断去除 DNA 之间的连接。在复制和分离之间存在大约 10-20 分钟的短暂时间差，在此期间，子染色体交织在一起。TopoIV 在细胞周期中的结合位置一直是争论的焦点。我们在这里表明，TopoIV 定位于分叉后尾随蛋白 SeqA 的原点近端侧，并遵循细胞中复制机制的运动模式。
