Hays E F, Kitada S, Uittenbogaart C H, Reeve J R
Laboratory of Biomedical and Environmental Sciences, Los Angeles, CA 90024.
J Natl Cancer Inst. 1988 Mar 16;80(2):116-21. doi: 10.1093/jnci/80.2.116.
By using an assay system in which small numbers of murine T lymphoma cells are stimulated to grow in serum-free medium, we have continued and expanded our previous studies of an autocrine growth factor that we call leukemia-derived growth factor (LDGF). We show that a T lymphoma cell line of immature phenotype, adapted to growth in serum-free medium, produces and responds to LDGF. LDGF activity is distinct from activities of 10 highly purified or recombinant hematopoietic growth factors including IL-1 and IL-2. However, growth-stimulating activity for the murine lymphoma cells is provided by a partially purified human LDGF.
通过使用一种检测系统，在该系统中少量鼠T淋巴瘤细胞被刺激在无血清培养基中生长，我们延续并扩展了我们之前对一种自分泌生长因子的研究，我们将其称为白血病衍生生长因子（LDGF）。我们表明，一种适应在无血清培养基中生长的未成熟表型的T淋巴瘤细胞系产生并对LDGF作出反应。LDGF活性与10种高度纯化或重组的造血生长因子（包括IL-1和IL-2）的活性不同。然而，鼠淋巴瘤细胞的生长刺激活性由部分纯化的人LDGF提供。
