Fisher G D, Kilgore W W
Department of Environmental Toxicology, University of California, Davis.
J Toxicol Environ Health. 1988;23(2):171-82. doi: 10.1080/15287398809531104.
Rats were exposed by inhalation to 1,3-dichloropropene (DCP) to assess the relationship between DCP exposure concentration and tissue levels of reduced glutathione (GSH). Animals were exposed for 1 h in a dynamic, nose-only system. GSH content, indicative of DCP metabolism, was measured in heart, kidney, liver, lung, nasal mucosa, and testes. A decrease in nasal GSH content was first seen at 5 ppm DCP and followed an exposure concentration-dependent curve. Exposure to concentrations above 305 ppm DCP reduced the level of liver GSH in an exposure concentration-dependent manner. Although depressed, lung GSH content remained relatively constant at approximately 75% of control following concentrations of up to 955 ppm DCP. Significant decreases in GSH content were observed in heart, liver, and testes only at 1716 ppm. Additional measurements were taken to investigate DCP distribution and potential indicators of acute toxicity. DCP was not present in the blood of animals 2 h after exposure to 955 ppm DCP or less. Serum lactate dehydrogenase activity was affected only at the highest exposure concentration, 1716 ppm DCP. Lung weight, measured at 2 and 6 h after exposure, did not differ from control for any of the exposure levels. This information demonstrated the importance of nasal tissue GSH in the metabolism of at least low levels of DCP. It also suggests the complexities involved with in vivo defence against inhaled DCP.
通过吸入法让大鼠接触1,3 - 二氯丙烯（DCP），以评估DCP接触浓度与还原型谷胱甘肽（GSH）组织水平之间的关系。动物在动态、仅经鼻的系统中暴露1小时。在心脏、肾脏、肝脏、肺、鼻黏膜和睾丸中测量了指示DCP代谢的GSH含量。在5 ppm DCP时首次观察到鼻内GSH含量下降，且呈接触浓度依赖性曲线。接触高于305 ppm DCP的浓度会以接触浓度依赖性方式降低肝脏GSH水平。尽管有所降低，但在高达955 ppm DCP的浓度下，肺GSH含量相对保持恒定，约为对照组的75%。仅在1716 ppm时，心脏、肝脏和睾丸中的GSH含量出现显著下降。还进行了额外测量以研究DCP分布和急性毒性的潜在指标。接触955 ppm DCP或更低浓度的动物在接触2小时后血液中未检测到DCP。血清乳酸脱氢酶活性仅在最高接触浓度1716 ppm DCP时受到影响。在接触后2小时和6小时测量的肺重量，在任何接触水平下与对照组均无差异。这些信息证明了鼻组织GSH在至少低水平DCP代谢中的重要性。这也表明了体内抵御吸入DCP所涉及的复杂性。
