Glucose-6-Phosphatase and α-Glucosidase Inhibitors from Roots Identified by Affinity-Directed Fractionation.

UNLABELLED

The ethanol/water-soluble extracts of the roots of M. Martens & Galeotti, Smilacaceae, which have been appreciated since pre-Hispanic times and traditionally used to treat type 2 diabetes in Mexico, were fractionated by the application of the affinity-directed method to identify glucose-6-phosphatase and α-glucosidase inhibitors. Mass spectrometry was used to identify the inhibitor as free ligands after released from the enzymatic complex by denaturing acidic conditions. The affinity study led to the identification of chlorogenic acid as a glucose-6-phosphatase inhibitor, which is the most abundant metabolite present in the analyzed crude drug. In addition, the affinity studies led to the identification of a flavonolignan glycoside as an α-glucosidase inhibitor. analysis with α-glucosidase MAL12 from the yeast allowed to determine the best conformational rearrangement for the α-glucosidase inhibitors. Graphical abstract.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s43450-020-00116-3.