Rosas-Ramírez Daniel, Arreguín-Espinosa Roberto, Escandón-Rivera Sonia, Andrade-Cetto Adolfo, Mata-Torres Gerardo, Pérez-Solís Ricardo
Departamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, Circuito Exterior s/n, Coyoacán, Ciudad Universitaria, Mexico City 04510, Mexico.
Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Av. Universidad 3000, Circuito Exterior s/n, Coyoacán, Ciudad Universitaria, Mexico City 04510, Mexico.
Plants (Basel). 2024 Feb 26;13(5):644. doi: 10.3390/plants13050644.
In the pursuit of identifying the novel resin glycoside modulators glucose-6-phosphatase and -glucosidase enzymes, associated with blood sugar regulation, methanol-soluble extracts from the flowers of (cazahuate, Nahuatl), renowned for its abundance of glycolipids, were employed. The methanol-soluble extracts were fractionated by applying the affinity-directed method with glucose-6-phosphatase enzymes from a rat's liver and -glucosidase enzymes from its intestines. Mass spectrometry and nuclear magnetic resonance were employed to identify the high-affinity compound as a free ligand following the release from the enzymatic complex. Gel permeation through a spin size-exclusion column allowed the separated high-affinity molecules to bind to glucose-6-phosphatase and -glucosidase enzymes in solution, which led to the identification of some previously reported resin glycosides in the flowers of cazahuate, where a glycolipid mainly structurally related to murucoidin XIV was observed. In vitro studies demonstrated the modulating properties of resin glycosides on the glucose-6-phosphatase enzyme. Dynamic light scattering revealed conformational variations induced by resin glycosides on -glucosidase enzyme, causing them to become more compact, akin to observations with the positive control, acarbose. These findings suggest that resin glycosides may serve as a potential source for phytotherapeutic agents with antihyperglycemic properties.
为了寻找与血糖调节相关的新型树脂糖苷调节剂——葡萄糖-6-磷酸酶和葡萄糖苷酶，研究人员采用了来自以富含糖脂而闻名的（纳瓦特语称卡扎瓦特）花朵的甲醇提取物。甲醇提取物通过采用来自大鼠肝脏的葡萄糖-6-磷酸酶和来自其肠道的葡萄糖苷酶的亲和导向方法进行分级分离。在从酶复合物中释放后，采用质谱和核磁共振来鉴定高亲和力化合物为游离配体。通过旋转尺寸排阻柱进行凝胶渗透，使分离出的高亲和力分子与溶液中的葡萄糖-6-磷酸酶和葡萄糖苷酶结合，这导致在卡扎瓦特花中鉴定出一些先前报道的树脂糖苷，其中观察到一种主要在结构上与murucoidin XIV相关的糖脂。体外研究证明了树脂糖苷对葡萄糖-6-磷酸酶的调节特性。动态光散射揭示了树脂糖苷对葡萄糖苷酶诱导的构象变化，使其变得更加紧凑，类似于阳性对照阿卡波糖的观察结果。这些发现表明，树脂糖苷可能成为具有降血糖特性的植物治疗剂的潜在来源。
