Torres Kamil, Pietrzyk Łukasz, Denisow-Pietrzyk Marta, Radej Sebastian, ChroŚcicki Andrzej, Torres Anna
Department of Didactics and Medical Simulation, Chair of Human Anatomy, Medical University of Lublin, Poland.
Laboratory of Biostructure, Chair and Department of Human Anatomy, Medical University of Lublin, Poland.
Cent Eur J Immunol. 2020;45(3):310-314. doi: 10.5114/ceji.2020.101255. Epub 2020 Nov 1.
Surgical intervention affects local and systemic immune responses, especially in obese individuals. Many studies have attempted to evaluate immunological response to surgical trauma. Surgery changes the quantity and phenotype of circulating blood dendritic cells (DCs), including a decrease of total DCs post-operatively. The study aimed to evaluate the percentage and changes of myeloid, lymphoid DCs, and myeloid to lymphoid DCs ratio in obese and normal weight patients undergoing laparoscopy.
The study enrolled asymptomatic patients with gallstones, who underwent laparoscopic cholecystectomy. Blood samples were obtained before the surgery as well as 24 and 48 hours after the surgery. Cells were collected using a FACSCalibur flow cytometry, and phenotypes were analyzed with CellQuest software.
No statistically significant differences were observed between obese and normal-weighted patients in all studied time periods, except for the myeloid to lymphoid DCs ratio assessed at 48-post-operative hour. The myeloid DCs percentage increased significantly in the post-operative period within both studied groups. The percentage of lymphoid DCs increased significantly in obese patients in all studied time periods.
Laparoscopy induces immunomodulation, such as changes of myeloid and lymphoid dendritic cells, especially in obese patients. We describe new findings, in which minimally invasive surgical trauma promotes the increase of percentage of circulating DCs in the early post-operative period.
手术干预会影响局部和全身免疫反应，尤其是在肥胖个体中。许多研究试图评估对手术创伤的免疫反应。手术会改变循环血液中树突状细胞（DCs）的数量和表型，包括术后总DCs数量减少。本研究旨在评估接受腹腔镜手术的肥胖和正常体重患者中髓样、淋巴样DCs的百分比及变化，以及髓样与淋巴样DCs的比例。
本研究纳入了无症状胆结石患者，他们接受了腹腔镜胆囊切除术。在手术前以及手术后24小时和48小时采集血样。使用FACSCalibur流式细胞仪收集细胞，并用CellQuest软件分析表型。
在所有研究时间段内，肥胖和正常体重患者之间未观察到统计学上的显著差异，但在术后48小时评估的髓样与淋巴样DCs比例除外。在两个研究组中，术后髓样DCs百分比均显著增加。在所有研究时间段内，肥胖患者的淋巴样DCs百分比均显著增加。
腹腔镜手术会诱导免疫调节，如髓样和淋巴样树突状细胞的变化，尤其是在肥胖患者中。我们描述了新的发现，即微创外科创伤在术后早期促进循环DCs百分比增加。
