基于干细胞的疗法对中风小鼠星形胶质细胞增生的影响。
Effect of stem cells-based therapy on astrogliosis in stroke subjected-mice.
作者信息
Akhoundzadeh Kobra, Vakili Abedin
机构信息
Faculty of Nursing, Qom University of Medical Sciences, Qom, Iran.
Physiology Research Center, Semnan University of Medical Sciences, Semnan, Iran.
出版信息
Stem Cell Investig. 2020 Dec 15;7:21. doi: 10.21037/sci-2020-031. eCollection 2020.
This study was planned to continue our previous study to assess effect of combination therapy bone marrow stromal cells (BMSCs) with exercise (EX) and triiodothyronine (T) on stroke-induced astrogliosis in mice. Stroke subjected-mice were divided into five monotherapy groups including sham, control, BMSCs, EX and T; and three combination therapy groups including BMSCs + EX, BMSCs + T and BMSCs + EX + T. Astrogliosis was assessed in ipsilateral hemisphere at day 7 after MCAO. Combination therapy BMSCs with EX and T could significantly decrease stroke-induced astrogliosis. However, monotherapy with BMSCs or EX also improved changes of glial fibrillary acidic protein (GFAP)-positive cells following stroke. Combination therapy BMSCs with EX and T didn't have any added effect on astrogliosis compared to monotherapy with BMSCs or EX. With comparing the present findings with the results of neurobehavioral functioning in our earlier study, it seems that decrease of astrogliosis could be helpful for stroke recovery.
本研究旨在延续我们之前的研究，评估骨髓基质细胞（BMSCs）联合运动（EX）和三碘甲状腺原氨酸（T）对小鼠脑卒中诱导的星形胶质细胞增生的影响。将脑卒中小鼠分为五个单一治疗组，包括假手术组、对照组、BMSCs组、EX组和T组；以及三个联合治疗组，包括BMSCs + EX组、BMSCs + T组和BMSCs + EX + T组。在大脑中动脉闭塞（MCAO）后第7天，对同侧半球的星形胶质细胞增生进行评估。BMSCs联合EX和T可显著降低脑卒中诱导的星形胶质细胞增生。然而，BMSCs或EX单一治疗也改善了脑卒中后胶质纤维酸性蛋白（GFAP）阳性细胞的变化。与BMSCs或EX单一治疗相比，BMSCs联合EX和T对星形胶质细胞增生没有额外影响。将本研究结果与我们早期研究中的神经行为功能结果进行比较，似乎星形胶质细胞增生的减少可能有助于脑卒中恢复。
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