Department of Gynecology and Obstetrics, Odense University Hospital, Odense, Denmark.
Department of Clinical Research, University of Southern Denmark, Odense, Denmark.
Basic Clin Pharmacol Toxicol. 2021 May;128(5):709-715. doi: 10.1111/bcpt.13558. Epub 2021 Jan 26.
The metabolite of ethanol, ethyl glucuronide (EtG), reflects alcohol intake longer than ethanol and is used as a biomarker in clinical settings to detect alcohol use. We aimed to assess the clinical usefulness in a low-to-moderate alcohol intake setting and validate a new urine EtG dipstick. A three-way, open, cross-over trial was conducted. Data were collected from January to June 2019. Among 12 healthy female volunteers, we quantified urine EtG and used a dipstick following intake of either one, two or four units of alcohol. Main outcomes were concentrations of EtG in urine and serum, and creatinine and ethanol in serum. EtG in urine was determined dichotomously by dipsticks at two different thresholds and by mass spectrometry used as gold standard. EtG in urine was quantifiable up to 24 hours after alcohol intake. In some individual cases, EtG was quantifiable up to 72 hours at low concentrations. The dipstick detected EtG in urine up to 24 hours. At thresholds of 1000 and 1500 ng/mL, the dipsticks had a specificity of 100% (both), while sensitivity was 84% and 69%, respectively. The sensitivity of the dipsticks was insufficient to support a screening purpose in this setting of low-to-moderate alcohol intake.
乙醇的代谢物乙基葡萄糖醛酸苷（EtG）反映了饮酒时间比乙醇更长，并且被用作临床环境中检测酒精使用的生物标志物。我们旨在评估在低至中度饮酒量环境中的临床实用性，并验证一种新的尿 EtG 检测棒。进行了一项三向、开放、交叉试验。数据收集于 2019 年 1 月至 6 月。在 12 名健康女性志愿者中，我们在摄入 1 个、2 个或 4 个单位的酒精后，定量检测了尿液中的 EtG 并使用了检测棒。主要结局指标是尿液和血清中的 EtG 浓度以及血清中的肌酐和乙醇浓度。尿液中的 EtG 用两种不同的检测棒进行二项式检测，并使用质谱法作为金标准进行定量检测。在饮酒后 24 小时内可定量检测到尿液中的 EtG。在某些个体病例中，低浓度时可在 72 小时内定量检测到 EtG。检测棒可在 24 小时内检测到尿液中的 EtG。在 1000 和 1500ng/mL 的阈值下，检测棒的特异性均为 100%（均），而敏感性分别为 84%和 69%。在这种低至中度饮酒量的情况下，检测棒的敏感性不足以支持筛查目的。
