Zhang Jun, He Lei, Wei Guo, Jiang Xuefeng, Fu Lei, Zhao Yue, Zhang Luxia, Yang Lutao, Li Yajuan, Wang Yutong, Mo Hong, Shen Jian
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Engineering Research Center for Biomedical Function Materials, School of Chemistry and Materials Science, Nanjing Normal University, Wenyuan Road #1, Xianlin University Town, Qixia District, Nanjing 210023, Jiangsu Province, China.
College of Materials Science and Engineering, Nanjing Forestry University, Longpan Road #159, Xuanwu District, Nanjing 210037, Jiangsu Province, China.
ACS Biomater Sci Eng. 2019 Sep 9;5(9):4366-4375. doi: 10.1021/acsbiomaterials.9b00684. Epub 2019 Aug 7.
More than 10 million people suffer from cardiovascular diseases, and diseased blood vessels need to be treated with vascular patches. For a vascular patch, good affinity for endothelial progenitor cells is a key factor in promoting the formation of endothelial tissue-endothelialization. To construct such a vascular patch with good cell affnity, in this work, we first synthesized a reactive zwitterionic organophosphate containing a phosphorylcholine headgroup: 6-(acryloyloxy)hexyl-2-(-isopropyl-,-dimethylammonio)ethyl phosphate (AHEP). We then grafted AHEP onto a polylactic acid (PLA)-coated decellularized scaffold to obtain a vascular patch. Its hydrophilicity and biocompatibility were investigated. Its in vivo performance was also examined in a pig model with B-ultrasonography, Doppler spectra, and computed tomography angiography. The vascular patch demonstrated a nonhemolytic property, noncytotoxicity, long in vitro coagulation times, the strong ability to resist platelet adhesion, and a good affinity for endothelial progenitor cells. The vascular patch was able to maintain the long-term patency (5 months) of surgical arteries. Hence, the zwitterionic polymer-grafted PLA vascular patch may be a promising candidate for vascular tissue engineering.
超过1000万人患有心血管疾病，患病血管需要用血管补片进行治疗。对于血管补片而言，对内皮祖细胞具有良好的亲和力是促进内皮组织形成——内皮化的关键因素。为构建具有良好细胞亲和力的这种血管补片，在本研究中，我们首先合成了一种含有磷酰胆碱头基的反应性两性离子有机磷酸酯：6-(丙烯酰氧基)己基-2-(-异丙基-,-二甲基铵)乙基磷酸酯(AHEP)。然后我们将AHEP接枝到聚乳酸(PLA)涂层的脱细胞支架上以获得血管补片。对其亲水性和生物相容性进行了研究。还通过B超、多普勒光谱和计算机断层血管造影在猪模型中检查了其体内性能。该血管补片表现出非溶血特性、无细胞毒性、体外凝血时间长、抗血小板粘附能力强以及对内皮祖细胞具有良好的亲和力。该血管补片能够维持手术动脉的长期通畅(5个月)。因此，两性离子聚合物接枝的PLA血管补片可能是血管组织工程中有前景的候选材料。
