Department of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China.
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
ACS Appl Bio Mater. 2023 Mar 20;6(3):1071-1080. doi: 10.1021/acsabm.2c00957. Epub 2023 Mar 6.
Vascular transplantation is an effective strategy against cardiovascular diseases (CVD), and artificial vascular patches are of urgent need across the world. In this work, we designed a multifunctional decellularized scaffolds (DCS)-based vascular patch for porcine vascular repair. Ammonium phosphate zwitter-ion (APZI) and poly(vinyl alcohol) (PVA) hydrogel were coated on the surface of DCS to improve the mechanical properties and biocompatibility of an artificial vascular patch. Then a heparin (Hep)-loaded metal-organic framework (MOF) further decorated the artificial vascular patches to inhibit blood coagulation and promote vascular endothelialization. The obtained artificial vascular patch showed suitable mechanical properties, good biocompatibility, and blood compatibility. In addition, the proliferation and adhesion of endothelial progenitor cells (EPCs) on the surface of artificial vascular patch improved a lot when compared with unmodified PVA/DCS. According to the results of B-ultrasound and CT images, the artificial vascular patch could maintain the patency of the implant site after implanting into the pig carotid artery. The current results solidly support that a MOF-Hep/APZI-PVA/DCS vascular patch would be an excellent vascular replacement material.
血管移植是治疗心血管疾病（CVD）的有效策略，全世界都急需人工血管补片。在这项工作中，我们设计了一种基于多功能脱细胞支架（DCS）的血管补片，用于猪血管修复。在 DCS 表面涂覆了磷酸铵两性离子（APZI）和聚乙烯醇（PVA）水凝胶，以提高人工血管补片的机械性能和生物相容性。然后，进一步修饰人工血管补片以负载肝素（Hep）的金属有机骨架（MOF），以抑制血液凝固和促进血管内皮化。所得到的人工血管补片具有合适的机械性能、良好的生物相容性和血液相容性。此外，与未改性的 PVA/DCS 相比，人工血管补片表面内皮祖细胞（EPC）的增殖和黏附能力有了很大提高。根据 B 超和 CT 图像的结果，人工血管补片在植入猪颈动脉后能保持植入部位的通畅。目前的结果有力地支持了 MOF-Hep/APZI-PVA/DCS 血管补片将是一种优秀的血管替代材料。
