Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Biomed Phys Eng Express. 2020 Mar 25;6(3):035011. doi: 10.1088/2057-1976/ab7284.
Bone injury, especially bone damages due to the removal of bone tumors, is one of the most important issues in the field of therapeutic research in tissue engineering applications. In this context, ceramic-based composites have attracted widespread attention since they have mechanical properties close to the natural bone, hence providing similar conditions for the extracellular matrix (ECM). Thus, in this study, hardystonite and diopside (HT-Di) scaffolds containing various diopside amounts from 5 to 25 wt% were prepared by the space holder method. The results revealed that the fabricated scaffolds contain 70%-75% porosity with a pore size of 300-500 μm and a compressive strength of about 0.54 to 1.71 MPa which is perfectly in the range of the compressive strength of the sponge bone. Noticeably, great apatite formation ability was observed in the scaffold with diopside, although the scaffold without diopside showed poor bioactivity. The MTT assay depicted that the inclusion of diopside into hardystonite scaffold resulted in dramatic enhancement in the MG-63 cell viability. Moreover, the scaffold with diopside offered greater cell attachment and spreading than the scaffold without diopside. Therefore, the synergistic effects of the scaffold with 12.5 wt% of diopside, including great mechanical characteristic, excellent bioactivity, and appealing biocompatibility enable it to be an appealing choice for bone tissue engineering applications.
骨损伤，尤其是因骨肿瘤切除而导致的骨损伤，是组织工程应用治疗研究领域的最重要问题之一。在这种情况下，基于陶瓷的复合材料由于具有与天然骨相近的机械性能，从而为细胞外基质（ECM）提供了相似的条件，因此受到了广泛关注。因此，在这项研究中，采用空间占位剂法制备了含有不同含量（5-25wt%）透辉石的硬硅钙石和透辉石（HT-Di）支架。结果表明，所制备的支架具有 70%-75%的孔隙率，孔径为 300-500μm，抗压强度约为 0.54-1.71MPa，完全处于海绵骨抗压强度范围内。值得注意的是，虽然不含透辉石的支架表现出较差的生物活性，但含透辉石的支架具有很强的磷灰石形成能力。MTT 试验表明，将透辉石掺入硬硅钙石支架中可显著提高 MG-63 细胞的活力。此外，含透辉石的支架比不含透辉石的支架具有更好的细胞黏附和铺展能力。因此，含 12.5wt%透辉石的支架具有出色的机械特性、优异的生物活性和良好的生物相容性，是骨组织工程应用的理想选择。
