Bagherpour Iman, Yaghtin Amirhossein, Naghib Seyed Morteza, Molaabasi Fatemeh
Department of Materials Science and Engineering, College of Engineering No.2, Islamic Azad University, Shiraz branch, Iran.
Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology (IUST), Tehran, Iran.
Front Bioeng Biotechnol. 2023 Mar 13;11:1073435. doi: 10.3389/fbioe.2023.1073435. eCollection 2023.
The use of hardystonite (CaZnSiO, HT)-based composites could be one the main strategies to improve mechanical properties closing to natural bone. However, there are a few reports in this regard. Recent findings indicate that graphene is a promising biocompatible additive in ceramic-based composite. Here, we propose a simple approach for the synthesis of porous nano- and microstructured hardystonite/reduced graphene oxide (HT/RGO) composite using a sol-gel method followed by ultrasonic and hydrothermal processes. Integrating GO to the pure HT increased the bending strength and toughness values about 27.59% and 34.33%, respectively. It also allowed the increment of compressive strength and compressive modulus by about 8.18% and 86%, respectively, and improvement in the fracture toughness about 11.8 times compared to pure HT. The formation of HT/RGO nanocomposites with different RGO weight percentages ranging from 0 to 5.0 has been investigated by scanning electron microscopy (SEM) and X-ray diffraction and the efficient incorporation of GO nanosheets into HT nanocomposite as well as the mesoporous structural properties were also confirmed by Raman, FTIR and BET analyses. The cell viability of HT/RGO composite scaffolds was assayed by methyl thiazole tetrazolium (MTT) test . In this regard, the alkaline phosphatase (ALP) activity and the proliferation rate of mouse osteoblastic cells (MC3T3-E1) on the HT/1 wt. % RGO composite scaffold enhanced in comparison with the pure HT ceramic. The adhesion of osteoblastic cells on the 1% wt. HT/RGO scaffold was interesting as well. In addition, the effect of 1% wt. HT/RGO extract on the proliferation of osteoblast human G-292 cells was successfully evaluated and remarkable observations were obtained. All together it can be said that the proposed bioceramic hardystonite/reduced graphene oxide composites can be a promising candidate for designing hard tissue implants.
使用基于钙锌黄长石（CaZnSiO₄，HT）的复合材料可能是提高力学性能以接近天然骨的主要策略之一。然而，这方面的报道较少。最近的研究结果表明，石墨烯是陶瓷基复合材料中一种很有前景的生物相容性添加剂。在此，我们提出一种简单的方法，通过溶胶 - 凝胶法，随后进行超声和水热过程，合成多孔纳米和微结构的钙锌黄长石/还原氧化石墨烯（HT/RGO）复合材料。将氧化石墨烯（GO）与纯HT结合后，弯曲强度和韧性值分别提高了约27.59%和34.33%。它还使抗压强度和抗压模量分别提高了约8.18%和86%，与纯HT相比，断裂韧性提高了约11.8倍。通过扫描电子显微镜（SEM）和X射线衍射研究了不同RGO重量百分比（范围从0到5.0）的HT/RGO纳米复合材料的形成，拉曼光谱、傅里叶变换红外光谱（FTIR）和比表面积分析仪（BET）分析也证实了GO纳米片有效掺入HT纳米复合材料以及介孔结构特性。通过甲基噻唑四氮唑（MTT）试验测定了HT/RGO复合支架的细胞活力。在这方面，与纯HT陶瓷相比，小鼠成骨细胞（MC3T3 - E1）在HT/1 wt.% RGO复合支架上的碱性磷酸酶（ALP）活性和增殖率有所提高。成骨细胞在1% wt. HT/RGO支架上的黏附情况也很有趣。此外，成功评估了1% wt. HT/RGO提取物对人成骨细胞G - 292细胞增殖的影响，并获得了显著的观察结果。总之，可以说所提出的生物陶瓷钙锌黄长石/还原氧化石墨烯复合材料可能是设计硬组织植入物的有前途的候选材料。
