Ayala S, Rodríguez-Del Valle N
Department of Microbiology & Medical Zoology, University of Puerto Rico, San Juan.
Mycopathologia. 1988 Feb;101(2):113-20. doi: 10.1007/BF00452896.
Hyaline, non pigmented microconidia of Sporothrix schenckii were harvested and allowed to form germ tubes in a basal medium with glucose at pH 4.0 and 25 degrees C. These conditions supported only the development of the mycelial form of Sporothrix schenckii in a reproducible, synchronized manner which allowed further analysis of the early cellular events occurring during the germination of the conidia. The relationship between macro-molecular synthesis (DNA, RNA and protein synthesis) and nuclear division, hyphal growth and septum formation were established. Following inoculation, protein synthesis was observed after 10 minutes followed by RNA synthesis, after 1 h and DNA synthesis after 2 h. The first nuclear division was observed during the 9 to 12 h interval after inoculation. Germ tube formation slightly preceeded nuclear division and was first evidenced 9 h after the induction of germination but was not completed until 12 h after inoculation. Septation was first observed in the germ tubes 0.25 micron from the mother cell-germ tube function 9 h after induction of germination.
收集申克孢子丝菌的透明、无色素小分生孢子，并使其在pH值为4.0、含葡萄糖的基础培养基中于25℃形成芽管。这些条件仅以可重复、同步的方式支持申克孢子丝菌菌丝体形态的发育，从而能够进一步分析分生孢子萌发过程中发生的早期细胞事件。确定了大分子合成（DNA、RNA和蛋白质合成）与核分裂、菌丝生长和隔膜形成之间的关系。接种后，10分钟后观察到蛋白质合成，1小时后观察到RNA合成，2小时后观察到DNA合成。首次核分裂在接种后9至12小时之间观察到。芽管形成略先于核分裂，在诱导萌发后9小时首次出现，但直到接种后12小时才完成。在诱导萌发后9小时，在距母细胞-芽管连接处0.25微米的芽管中首次观察到隔膜形成。
