Kuo Che-Ying, Eranki Avinash, Placone Jesse K, Rhodes Kelly R, Aranda-Espinoza Helim, Fernandes Rohan, Fisher John P, Kim Peter C W
Fischell Department of Bioengineering, University of Maryland College Park, 8228 Paint Branch Drive, College Park, Maryland 20742, United States.
Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, 111 Michigan Avenue NW, Washington, D.C. 20010, United States.
ACS Biomater Sci Eng. 2016 Oct 10;2(10):1817-1826. doi: 10.1021/acsbiomaterials.6b00031. Epub 2016 May 6.
Preeclampsia (PE) is a leading cause of maternal and perinatal morbidity and mortality. Current research suggests that the impaired trophoblastic invasion of maternal spiral arteries contributes significantly to the development of PE. However, the pathobiology of PE remains poorly understood, and there is a lack of treatment options largely due to ineffective experimental models. Utilizing the capability of bioprinting and shear wave elastography, we developed a 3D, bioengineered placenta model (BPM) to study and quantify cell migration. Through BPM, we evaluated the effect of epidermal growth factor (EGF) on the migratory behavior of trophoblast and human mesenchymal stem cells. Our results demonstrate a positive correlation between cell migration rates and EGF concentration. These results indicate that a feasible placental model can be bioprinted to examine cellular, molecular, and pharmacologic interactions. In addition, EGF clearly affects the celluar migration, a potential therapeutic agent to treat preeclampsia. We envision that our tissue modeling approach can be readily transferred to study other normal biologic and abnormal pathologic processes such as fibroblast migration in wound healing and stem cell homing.
子痫前期（PE）是孕产妇和围产儿发病及死亡的主要原因。目前的研究表明，滋养层细胞对母体螺旋动脉的侵入受损是子痫前期发展的重要原因。然而，子痫前期的病理生物学仍知之甚少，而且由于实验模型效果不佳，缺乏有效的治疗方案。利用生物打印和剪切波弹性成像技术，我们开发了一种三维生物工程胎盘模型（BPM）来研究和量化细胞迁移。通过BPM，我们评估了表皮生长因子（EGF）对滋养层细胞和人骨髓间充质干细胞迁移行为的影响。我们的结果表明细胞迁移率与EGF浓度呈正相关。这些结果表明，可以通过生物打印构建一个可行的胎盘模型来研究细胞、分子和药物相互作用。此外，EGF明显影响细胞迁移，是一种治疗子痫前期的潜在治疗药物。我们设想，我们的组织建模方法可以很容易地应用于研究其他正常生物学和异常病理过程，如伤口愈合中的成纤维细胞迁移和干细胞归巢。
