人诱导多能干细胞衍生的神经干细胞在含有连续浓度梯度的β-连环蛋白衍生肽His-Ala-Val-Asp-Ile的聚乙二醇二甲基丙烯酸酯水凝胶上的存活和神经分化
Human Induced Pluripotent Stem Cell Derived Neural Stem Cell Survival and Neural Differentiation on Polyethylene Glycol Dimethacrylate Hydrogels Containing a Continuous Concentration Gradient of -Cadherin Derived Peptide His-Ala-Val-Asp-Ile.
作者信息
Lim Hyun Ju, Khan Zara, Wilems Thomas S, Lu Xi, Perera T Hiran, Kurosu Yuki E, Ravivarapu Krishna T, Mosley Matthew C, Smith Callahan Laura A
机构信息
The Vivian L. Smith Department of Neurosurgery and Center for Stem Cells and Regenerative Medicine, McGovern Medical School at the University of Texas Health Science Center at Houston, 1825 Pressler, Houston, Texas 77030, United States.
Department of Nanomedicine and Biomedical Engineering, McGovern Medical School at the University of Texas Health Science Center at Houston, 1825 Pressler, Houston, Texas 77030, United States.
出版信息
ACS Biomater Sci Eng. 2017 May 8;3(5):776-781. doi: 10.1021/acsbiomaterials.6b00745. Epub 2017 Apr 18.
Although preclinical models of spinal cord injury have shown that matrix inclusion in stem cell therapy leads to greater neurological improvements than that including cells alone, there has been insufficient matrix optimization for human cells. -Cadherin influences the development and maintenance of neural tissue, but the effects of -cadherin derived peptide His-Ala-Val-Asp-Ile (HAVDI) on the survival, neurite extension, and expression of neural differentiation markers in human induced pluripotent stem cell derived neural stems (hNSC) have not been widely examined. Using polyethylene glycol hydrogels containing a continuous gradient of HAVDI, this study identifies concentration dependent effects on hNSC survival and neural differentiation.
尽管脊髓损伤的临床前模型表明，在干细胞治疗中加入基质比单纯使用细胞能带来更大的神经功能改善，但针对人类细胞的基质优化仍不足。-钙黏蛋白影响神经组织的发育和维持，但-钙黏蛋白衍生肽His-Ala-Val-Asp-Ile（HAVDI）对人诱导多能干细胞衍生神经干细胞（hNSC）的存活、神经突延伸及神经分化标志物表达的影响尚未得到广泛研究。本研究使用含有连续梯度HAVDI的聚乙二醇水凝胶，确定了其对hNSC存活和神经分化的浓度依赖性影响。
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