Castro Antonio G B, Lo Giudice Maria Cristina, Vermonden Tina, Leeuwenburgh Sander C G, Jansen John A, van den Beucken Jeroen J J P, Yang Fang
Department of Biomaterials, Radboud University Medical Center, Philips van Leydenlaan 25, Nijmegen 6525 EX, The Netherlands.
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Faculty of Science, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands.
ACS Biomater Sci Eng. 2016 Nov 14;2(11):2099-2107. doi: 10.1021/acsbiomaterials.6b00522. Epub 2016 Oct 4.
A wide range of particles have been developed for different applications in drug-delivery, tissue engineering, or regenerative medicine. In contrast to traditional spherical particles, nonspherical (e.g., cylindrical) particles possess several structural and morphological advantages that make them attractive for specific applications. Here, we developed a top-down approach to process electrospun fibers into microsized cylinders (i.e., microcylinders) with high specific surface area and with or without surface porosity. To obtain these microcylinders, poly(l-lactic acid) (PLLA) solutions were subjected to electrospinning, followed by an aminolysis-based chemical scission procedure. The morphology, structure, and chemical composition of the microcylinders were then characterized. The specific surface area and surface porosity of the microcylinders were controlled by the volatility of the solvents, and their length was dependent on the duration of the aminolysis reaction. During aminolysis, the microcylinders became functionalized with amine groups, enabling potential further modifications by grafting with compounds containing desired chemical groups, for example, carboxyl, carbonyl, or hydroxyl functional groups. Additionally, the microcylinders showed in vitro biocompatible properties related to cell viability. These data demonstrate that PLLA microcylinders with high specific surface area, optional surface porosity, amine-based functional handles granting additional functionalization, and cytocompatible properties are candidate materials for future biomedical applications.
人们已经开发出各种各样的颗粒用于药物递送、组织工程或再生医学的不同应用。与传统的球形颗粒相比，非球形（如圆柱形）颗粒具有多种结构和形态优势，使其在特定应用中具有吸引力。在这里，我们开发了一种自上而下的方法，将电纺纤维加工成具有高比表面积且有或没有表面孔隙率的微米级圆柱体（即微圆柱体）。为了获得这些微圆柱体，将聚（L-乳酸）（PLLA）溶液进行静电纺丝，然后进行基于氨解的化学裂解程序。然后对微圆柱体的形态、结构和化学成分进行了表征。微圆柱体的比表面积和表面孔隙率由溶剂的挥发性控制，其长度取决于氨解反应的持续时间。在氨解过程中，微圆柱体被胺基官能化，通过与含有所需化学基团（例如羧基、羰基或羟基官能团）的化合物接枝，能够进行潜在的进一步修饰。此外，微圆柱体显示出与细胞活力相关的体外生物相容性。这些数据表明，具有高比表面积、可选表面孔隙率、赋予额外功能化的胺基功能手柄以及细胞相容性的PLLA微圆柱体是未来生物医学应用的候选材料。
