Choi Kwang-Min, Joo Min-Soo, Cho Dong-Hee, Woo Won-Sik, Kang Gyoungsik, Heo Min Jin, Kim Do-Hyung, Park Chan-Il
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Korea.
Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, 45, Yongso-ro, Nam-Gu., Busan 48513, Korea.
Animals (Basel). 2021 Jan 11;11(1):139. doi: 10.3390/ani11010139.
Galectin () is a member of a family of β-galactoside-binding lectin. The members of this family play important roles in the recognition of carbohydrate ligands and in various other biological processes. In this study, we identified the gene encoding in () and analyzed its expression in various tissues after pathogen challenge. Alignment analysis revealed that the two galactose-binding lectin domains of the deduced protein were highly conserved among all the teleosts. Phylogenetic analysis revealed that is most closely related to the gene of gilthead sea bream. was ubiquitously expressed in all tissues analyzed but was predominantly expressed in the spleen, head kidney, and intestine. After challenges with major microbial pathogens (, , or red sea bream iridovirus) of red sea bream, mRNA expression was significantly regulated in most immune-related tissues. These results suggested that not only plays an important role in the immune system of red sea bream but is also a possible inflammatory marker for pathogenic diseases.
半乳糖凝集素（）是β-半乳糖苷结合凝集素家族的成员。该家族成员在碳水化合物配体的识别及各种其他生物学过程中发挥重要作用。在本研究中，我们在（）中鉴定了编码的基因，并分析了病原体攻击后其在各种组织中的表达。比对分析表明，推导蛋白的两个半乳糖结合凝集素结构域在所有硬骨鱼中高度保守。系统发育分析表明，与金头鲷的基因关系最为密切。在所有分析的组织中均普遍表达，但在脾脏、头肾和肠道中表达尤为突出。在用真鲷的主要微生物病原体（、或真鲷虹彩病毒）攻击后，在大多数免疫相关组织中，mRNA表达受到显著调节。这些结果表明，不仅在真鲷的免疫系统中发挥重要作用，而且可能是致病疾病的炎症标志物。
