Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, Slovenia.
Veterinary Faculty, Institute of Preclinical Sciences, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia.
Molecules. 2021 Jan 11;26(2):345. doi: 10.3390/molecules26020345.
Intestinal transepithelial transport of glucose is mediated by glucose transporters, and affects postprandial blood-glucose levels. This study investigates the effect of wood extracts rich in hydrolyzable tannins (HTs) that originated from sweet chestnut ( Mill.) and oak () on the expression of glucose transporter genes and the uptake of glucose and HT constituents in a 3D porcine-small-intestine epithelial-cell model. The viability of epithelial cells CLAB and PSI exposed to different HTs was determined using alamarBlue. qPCR was used to analyze the gene expression of SGLT1, GLUT2, GLUT4, and POLR2A. Glucose uptake was confirmed by assay, and LC-MS/ MS was used for the analysis of HT bioavailability. HTs at 37 µg/mL were found to adversely affect cell viability and downregulate POLR2A expression. HT from wood extract Tanex at concentrations of 4 µg/mL upregulated the expression of GLUT2, as well as glucose uptake at 1 µg/mL. The time-dependent passage of gallic acid through enterocytes was influenced by all wood extracts compared to gallic acid itself as a control. These results suggest that HTs could modulate glucose uptake and gallic acid passage in the 3D cell model.
肠上皮细胞的葡萄糖转运是由葡萄糖转运蛋白介导的，这会影响餐后血糖水平。本研究探讨了富含水解单宁（HTs）的木材提取物对葡萄糖转运蛋白基因表达和 3D 猪小肠上皮细胞模型中葡萄糖和 HT 成分摄取的影响。用 alamarBlue 测定上皮细胞 CLAB 和 PSI 暴露于不同 HTs 时的活力。采用 qPCR 分析 SGLT1、GLUT2、GLUT4 和 POLR2A 的基因表达。通过测定证实葡萄糖摄取，并用 LC-MS/MS 分析 HT 的生物利用度。发现 37 µg/mL 的 HT 会对细胞活力产生不利影响，并下调 POLR2A 的表达。Tanex 木材提取物中的 HT 浓度为 4 µg/mL 时，会上调 GLUT2 的表达，以及 1 µg/mL 时的葡萄糖摄取。与作为对照的没食子酸本身相比，所有木材提取物都会影响没食子酸穿过肠细胞的时间依赖性传递。这些结果表明，HTs 可以调节 3D 细胞模型中的葡萄糖摄取和没食子酸传递。
