School of Life Science and Technology, Tokyo Institute of Technology, 4259, Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Department of Applied Chemistry, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
J Am Chem Soc. 2021 Jan 27;143(3):1348-1355. doi: 10.1021/jacs.0c09470. Epub 2021 Jan 14.
Transmembrane proteins within biological membranes exhibit varieties of important functions that are vital for many cellular activities, and the development of their synthetic mimetics allows for deep understanding in related biological events. Inspired by the structures and functions of natural ion channels that can respond to multiple stimuli in an anisotropic manner, we developed multiblock amphiphile in this study. When was incorporated into the lipid bilayer membranes, formed a supramolecular ion channel whose ion transport property was controllable by the polarity and amplitude of the applied voltage. Microscopic emission spectroscopy revealed that changed its molecular conformation in response to the applied voltage. Furthermore, the ion transport property of could be reversibly switched by the addition of ()-propranolol, an aromatic amine known as an antiarrhythmic agent, followed by the addition of β-cyclodextrin for its removal. The highly regulated orientation of allowed for an anisotropic dual-stimuli-responsiveness for the first time as a synthetic ion channel.
生物膜中的跨膜蛋白表现出多种重要功能，对许多细胞活动至关重要，而它们的合成类似物的开发可以深入了解相关的生物事件。受能够以各向异性方式响应多种刺激的天然离子通道的结构和功能的启发，我们在本研究中开发了多嵌段两亲物。当 被掺入脂质双层膜中时，形成了超分子离子通道，其离子传输性质可以通过施加电压的极性和幅度来控制。微观发射光谱表明， 响应施加的电压改变了其分子构象。此外，通过添加芳香胺已知的抗心律失常药物（）-普萘洛尔，可以可逆地切换 的离子传输性质，然后添加β-环糊精将其去除。 的高度调节取向首次允许作为合成离子通道的各向异性双重刺激响应。
