Yue B Y, Kurosawa A, Duvall J, Goldberg M F, Tso M O, Sugar J
Department of Ophthalmology, University of Illinois at Chicago, Lions of Illinois Eye Research Institute 60612.
Ophthalmology. 1988 Jan;95(1):56-60. doi: 10.1016/s0161-6420(88)33215-x.
The authors performed fibronectin studies on scleral specimens derived from a patient with nanophthalmos. Immunohistochemical staining with antifibronectin was conducted using both formaldehyde-fixed, paraffin-embedded tissue sections and unfixed tissue-cultured scleral cells. In each case, the nanophthalmic samples exhibited fibronectin staining stronger than that obtained from normal human subjects. Results from an enzyme-linked immunosorbent assay (ELISA) confirmed the histologic findings that, in tissue culture, the patient's scleral cells contained and secreted a higher amount of fibronectin than did the normal control cells. The elevated fibronectin level may be related to the development of nanophthalmos.
作者对取自一名小眼球症患者的巩膜标本进行了纤连蛋白研究。使用甲醛固定、石蜡包埋的组织切片和未固定的组织培养巩膜细胞进行抗纤连蛋白免疫组织化学染色。在每种情况下，小眼球症样本的纤连蛋白染色都比正常人类受试者的更强。酶联免疫吸附测定（ELISA）结果证实了组织学发现，即在组织培养中，患者的巩膜细胞比正常对照细胞含有并分泌更多的纤连蛋白。纤连蛋白水平升高可能与小眼球症的发生有关。
