Takumida M, Bagger-Sjöbäck D, Rask-Andersen H
Department of Otolaryngology, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.
Arch Otorhinolaryngol. 1988;245(5):266-72. doi: 10.1007/BF00464628.
The ultrastructure of the basement membrane of the murine endolymphatic sac was studied under various experimental conditions in labyrinthectomized and ethacrynic acid-treated animals and was compared with normal anatomy. The basement membrane was clearly visualized after staining with ruthenium red or dialyzed iron. The basement membrane of the murine sac consists of two different layers: the lamina rara and the lamina densa. It demarcates the border between the epithelial cells and the subepithelial connective tissue. Our findings suggest that the basement membrane acts as a physical support to the epithelium in the endolymphatic sac. The basement membrane also shows a dynamic capacity to form a new basement membrane, with the result that the lateral intercellular space between contiguous epithelial cells may be integrated with the subepithelial space. This system is believed to act as a macromolecular and bulk water transport system. In the subepithelial space, collagen, reticular and elastic fibrils are found having a close relationship to the basement membrane. The elastic fibres are presumed to play a role in the pressure-regulating mechanism in the endolymphatic sac.
在迷路切除和依他尼酸处理的动物中，于各种实验条件下研究了小鼠内淋巴囊基底膜的超微结构，并与正常解剖结构进行了比较。用钌红或透析铁染色后，基底膜清晰可见。小鼠囊的基底膜由两层不同的结构组成：透明层和致密层。它划分了上皮细胞和上皮下结缔组织之间的边界。我们的研究结果表明，基底膜对内淋巴囊中的上皮起到物理支撑作用。基底膜还具有形成新基底膜的动态能力，结果相邻上皮细胞之间的侧向细胞间隙可能与上皮下间隙整合。该系统被认为是一个大分子和大量水的运输系统。在上皮下间隙中，发现胶原纤维、网状纤维和弹性纤维与基底膜关系密切。推测弹性纤维在内淋巴囊的压力调节机制中发挥作用。
