Markiewicz P, Glucksmann A, Rothman-Denes L B
Department of Molecular Genetics, University of Chicago, IL 60637.
Nucleic Acids Res. 1988 Feb 11;16(3):1011-26. doi: 10.1093/nar/16.3.1011.
Bacteriophage N4 virion-encapsulated RNA polymerase, the enzyme responsible for transcription of the phage early RNAs, is unable to use duplex linear DNA as a template. In contrast to other RNA polymerases, the enzyme transcribes denatured N4 DNA with in vivo specificity. The promoter sequences for three sites of transcription initiation on the N4 genome have been determined and found to contain conserved sequences and two sets of inverted repeats. In order to define the minimal sequence requirements for N4 virion RNA polymerase activity, we have screened several heterologous DNAs, amounting to 64,328 bases, for their ability to support transcription. Several sequences allowing specific initiation were found. Their location, properties and the relation to N4 virion RNA polymerase promoters are discussed.
噬菌体N4病毒体包裹的RNA聚合酶负责噬菌体早期RNA的转录，该酶无法将双链线性DNA用作模板。与其他RNA聚合酶不同，该酶以体内特异性转录变性的N4 DNA。已确定N4基因组上三个转录起始位点的启动子序列，发现其包含保守序列和两组反向重复序列。为了确定N4病毒体RNA聚合酶活性的最小序列要求，我们筛选了总计64328个碱基的几种异源DNA，以检测它们支持转录的能力。发现了几个允许特异性起始的序列。讨论了它们的位置、特性以及与N4病毒体RNA聚合酶启动子的关系。
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