噬菌体N4病毒粒子RNA聚合酶催化结构域与单亚基RNA聚合酶相关。
The phage N4 virion RNA polymerase catalytic domain is related to single-subunit RNA polymerases.
作者信息
Kazmierczak K M, Davydova E K, Mustaev A A, Rothman-Denes L B
机构信息
Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, IL 60637, USA.
出版信息
EMBO J. 2002 Nov 1;21(21):5815-23. doi: 10.1093/emboj/cdf584.
Abstract
In vitro, bacteriophage N4 virion RNA polymerase (vRNAP) recognizes in vivo sites of transcription initiation on single-stranded templates. N4 vRNAP promoters are comprised of a hairpin structure and conserved sequences. Here, we show that vRNAP consists of a single 3500 amino acid polypeptide, and we define and characterize a transcriptionally active 1106 amino acid domain (mini-vRNAP). Biochemical and genetic characterization of this domain indicates that, despite its peculiar promoter specificity and lack of extensive sequence similarity to other DNA-dependent RNA polymerases, mini-vRNAP is related to the family of T7-like RNA polymerases.
摘要
在体外，噬菌体N4病毒粒子RNA聚合酶（vRNAP）可识别单链模板上体内转录起始位点。N4 vRNAP启动子由一个发夹结构和保守序列组成。在此，我们表明vRNAP由一条含3500个氨基酸的单多肽链组成，并且我们定义并表征了一个具有转录活性的1106个氨基酸的结构域（微型vRNAP）。对该结构域的生化和遗传学表征表明，尽管其启动子特异性独特且与其他依赖DNA的RNA聚合酶缺乏广泛的序列相似性，但微型vRNAP与T7样RNA聚合酶家族相关。
相似文献
1
The phage N4 virion RNA polymerase catalytic domain is related to single-subunit RNA polymerases.噬菌体N4病毒粒子RNA聚合酶催化结构域与单亚基RNA聚合酶相关。
EMBO J. 2002 Nov 1;21(21):5815-23. doi: 10.1093/emboj/cdf584.
2
Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase.噬菌体N4病毒粒子RNA聚合酶识别DNA发夹启动子的结构基础。
Mol Cell. 2008 Dec 5;32(5):707-17. doi: 10.1016/j.molcel.2008.11.010.
3
Identification of bacteriophage N4 virion RNA polymerase-nucleic acid interactions in transcription complexes.转录复合物中噬菌体N4病毒体RNA聚合酶与核酸相互作用的鉴定。
J Biol Chem. 2009 Jan 23;284(4):1962-70. doi: 10.1074/jbc.M807785200. Epub 2008 Nov 17.
4
Bacteriophage N4 virion RNA polymerase interaction with its promoter DNA hairpin.噬菌体N4病毒粒子RNA聚合酶与其启动子DNA发夹的相互作用。
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7033-8. doi: 10.1073/pnas.0610627104. Epub 2007 Apr 16.
5
Escherichia coli single-stranded DNA-binding protein mediates template recycling during transcription by bacteriophage N4 virion RNA polymerase.大肠杆菌单链DNA结合蛋白在噬菌体N4病毒粒子RNA聚合酶转录过程中介导模板循环利用。
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9250-5. doi: 10.1073/pnas.1133325100. Epub 2003 Jul 22.
6
Structural and biochemical investigation of bacteriophage N4-encoded RNA polymerases.噬菌体N4编码的RNA聚合酶的结构与生化研究。
Biomolecules. 2015 Apr 27;5(2):647-67. doi: 10.3390/biom5020647.
7
X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase.噬菌体N4病毒粒子RNA聚合酶聚合酶结构域的X射线晶体结构。
Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5046-51. doi: 10.1073/pnas.0712325105. Epub 2008 Mar 24.
8
Specific sequences and a hairpin structure in the template strand are required for N4 virion RNA polymerase promoter recognition.模板链中的特定序列和发夹结构是N4病毒粒子RNA聚合酶启动子识别所必需的。
Cell. 1992 Aug 7;70(3):491-500. doi: 10.1016/0092-8674(92)90173-a.
9
N4 virion DNA dependent-RNA polymerase: initiation sequences utilized by the enzyme on heterologous templates.N4病毒粒子DNA依赖性RNA聚合酶：该酶在异源模板上利用的起始序列。
Nucleic Acids Res. 1988 Feb 11;16(3):1011-26. doi: 10.1093/nar/16.3.1011.
10
N4 RNA polymerase II, a heterodimeric RNA polymerase with homology to the single-subunit family of RNA polymerases.N4 RNA聚合酶II，一种与单亚基RNA聚合酶家族具有同源性的异二聚体RNA聚合酶。
J Bacteriol. 2002 Sep;184(18):4952-61. doi: 10.1128/JB.184.18.4952-4961.2002.
引用本文的文献
1
Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor.整合结构分析揭示假单胞菌噬菌体 DEV 的基因组排出机制
Nat Commun. 2024 Oct 1;15(1):8482. doi: 10.1038/s41467-024-52752-1.
2
A Novel Podophage StenR_269 Suggests a New Family in the Class Caudoviricetes.一种新型噬藻体 stenR_269 提示在有尾噬菌体目新科的存在。
Viruses. 2023 Dec 15;15(12):2437. doi: 10.3390/v15122437.
3
A novel long-tailed myovirus represents a new T4-like cyanophage cluster.一种新型长尾肌病毒代表了一个新的类T4蓝藻噬菌体簇。
Front Microbiol. 2023 Nov 9;14:1293846. doi: 10.3389/fmicb.2023.1293846. eCollection 2023.
4
The Dynamics of Synthesis and Localization of Jumbo Phage RNA Polymerases inside Infected Cells.巨型噬菌体 RNA 聚合酶在感染细胞内的合成和定位动态。
Viruses. 2023 Oct 16;15(10):2096. doi: 10.3390/v15102096.
5
Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial .噬菌袋鼠：一种复杂的多糖去乙酰化尾丝，可作为果胶杆菌属中宿主识别的工具。
Int J Mol Sci. 2022 Sep 20;23(19):11043. doi: 10.3390/ijms231911043.
6
The structure and activities of the archaeal transcription termination factor Eta detail vulnerabilities of the transcription elongation complex.古菌转录终止因子 Eta 的结构和活性详细说明了转录延伸复合物的脆弱性。
Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2207581119. doi: 10.1073/pnas.2207581119. Epub 2022 Aug 2.
7
Tailed Lytic Bacteriophages of Soft Rot Pectobacteriaceae.软腐果胶杆菌科的尾状裂解噬菌体
Microorganisms. 2021 Aug 26;9(9):1819. doi: 10.3390/microorganisms9091819.
8
Intravirion DNA Can Access the Space Occupied by the Bacteriophage P22 Ejection Proteins.病毒粒子内的 DNA 可以进入噬菌体 P22 排出蛋白所占据的空间。
Viruses. 2021 Jul 30;13(8):1504. doi: 10.3390/v13081504.
9
Global Proteomic Profiling of Infection by a Giant Phage.巨型噬菌体感染的全局蛋白质组学分析。
J Virol. 2019 Feb 19;93(5). doi: 10.1128/JVI.01833-18. Print 2019 Mar 1.
10
Minimalism and functionality: Structural lessons from the heterodimeric N4 bacteriophage RNA polymerase II.极简主义和功能性：来自异二聚体 N4 噬菌体 RNA 聚合酶 II 的结构教训。
J Biol Chem. 2018 Aug 31;293(35):13616-13625. doi: 10.1074/jbc.RA118.003447. Epub 2018 Jul 10.
本文引用的文献
1
N4 RNA polymerase II, a heterodimeric RNA polymerase with homology to the single-subunit family of RNA polymerases.N4 RNA聚合酶II，一种与单亚基RNA聚合酶家族具有同源性的异二聚体RNA聚合酶。
J Bacteriol. 2002 Sep;184(18):4952-61. doi: 10.1128/JB.184.18.4952-4961.2002.
2
Effects of substitutions in a conserved DX(2)GR sequence motif, found in many DNA-dependent nucleotide polymerases, on transcription by T7 RNA polymerase.在许多依赖DNA的核苷酸聚合酶中发现的保守DX(2)GR序列基序中的取代对T7 RNA聚合酶转录的影响。
J Mol Biol. 2002 May 24;319(1):37-51. doi: 10.1016/S0022-2836(02)00261-9.
3
TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing.TREE-PUZZLE：使用四重奏和并行计算的最大似然系统发育分析。
Bioinformatics. 2002 Mar;18(3):502-4. doi: 10.1093/bioinformatics/18.3.502.
4
The genome of the natural genetic engineer Agrobacterium tumefaciens C58.天然遗传工程师根癌农杆菌C58的基因组。
Science. 2001 Dec 14;294(5550):2317-23. doi: 10.1126/science.1066804.
5
Roles of thiol-redox pathways in bacteria.硫醇-氧化还原途径在细菌中的作用。
Annu Rev Microbiol. 2001;55:21-48. doi: 10.1146/annurev.micro.55.1.21.
6
Identification and characterization of a Plasmodium falciparum RNA polymerase gene with similarity to mitochondrial RNA polymerases.一种与线粒体RNA聚合酶相似的恶性疟原虫RNA聚合酶基因的鉴定与特性分析。
Mol Biochem Parasitol. 2001 Apr 6;113(2):261-9. doi: 10.1016/s0166-6851(01)00223-7.
7
The conserved active site motif A of Escherichia coli DNA polymerase I is highly mutable.大肠杆菌DNA聚合酶I保守的活性位点基序A高度可变。
J Biol Chem. 2001 Jun 1;276(22):18836-42. doi: 10.1074/jbc.M011472200. Epub 2001 Mar 12.
8
DNA polymerase active site is highly mutable: evolutionary consequences.DNA聚合酶活性位点具有高度可变性：进化后果。
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5095-100. doi: 10.1073/pnas.97.10.5095.
9
RNA polymerase structure-function: insights into points of transcriptional regulation.RNA聚合酶的结构与功能：对转录调控关键点的见解
Curr Opin Microbiol. 2000 Apr;3(2):118-25. doi: 10.1016/s1369-5274(00)00062-x.
10
Structure of a transcribing T7 RNA polymerase initiation complex.转录中的T7 RNA聚合酶起始复合物的结构。
Science. 1999 Dec 17;286(5448):2305-9. doi: 10.1126/science.286.5448.2305.
Zotero 插件