Programa de Pós-Graduação em Microbiologia e Parasitologia Aplicadas, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Braz J Microbiol. 2021 Mar;52(1):185-193. doi: 10.1007/s42770-020-00415-w. Epub 2021 Jan 13.
Cystic fibrosis (CF) causes a variety of symptoms in different organs, but the majority of the morbidity and mortality of CF is related with pulmonary conditions. Primary infections are usually bacterial, and when treated with antibiotics, yeast infections appear or become more evident. Studies show that different microorganisms can co-inhabit the same environment and the interactions could be synergistic or antagonistic. Using techniques including viable and non-viable cell-to-cell interactions, mixed culture in liquid, and solid media sharing or not the supernatant, this study has evaluated interactions between the fungal species Scedosporium apiospermum and Scedosporium boydii with the bacterial species Staphylococcus aureus, Pseudomonas aeruginosa, and Burkholderia cepacia. Cell-to-cell interactions in liquid medium showed that P. aeruginosa and B. cepacia were able to reduce fungal viability but only in the presence of alive bacteria. Interactions without cell contact using a semi-permeable membrane showed that all bacteria were able to inhibit both fungal growths/viabilities. Cell-free supernatants from bacterial growth reduced fungal viability in planktonic fungal cells as well as in some conditions for preformed fungal biomass. According to the chemical analysis of the bacterial supernatants, the predominant component is protein. In this work, we verified that bacterial cells and their metabolites, present in the supernatants, can play anti-S. apiospermum and anti-S. boydii roles on fungal growth and viability.
囊性纤维化（CF）可导致不同器官出现多种症状，但 CF 的大多数发病率和死亡率与肺部疾病有关。原发性感染通常是细菌感染，经抗生素治疗后，真菌感染出现或变得更加明显。研究表明，不同的微生物可以共同栖息在同一环境中，其相互作用可能是协同的，也可能是拮抗的。本研究使用包括活细胞与死细胞之间的相互作用、液体和固体混合培养以及是否共享上清液等技术，评估了棘孢木霉菌和棘孢帚霉与金黄色葡萄球菌、铜绿假单胞菌和洋葱伯克霍尔德菌这三种细菌之间的相互作用。液体培养基中的细胞间相互作用表明，铜绿假单胞菌和洋葱伯克霍尔德菌能够降低真菌的活力，但仅在存在活细菌的情况下。使用半透膜进行无细胞接触的相互作用表明，所有细菌都能够抑制两种真菌的生长/活力。来自细菌生长的无细胞上清液降低了浮游真菌细胞以及某些条件下预先形成的真菌生物量中的真菌活力。根据细菌上清液的化学分析，主要成分是蛋白质。在这项工作中，我们验证了细菌细胞及其代谢物，存在于上清液中，可对真菌的生长和活力发挥抗棘孢木霉菌和抗棘孢帚霉的作用。
