Department of Microbiology, Key Laboratory of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
College of Resource and Environment, Anhui Science and Technology University, Anhui, 233100, China.
Ecotoxicology. 2021 Oct;30(8):1559-1571. doi: 10.1007/s10646-020-02336-8. Epub 2021 Jan 14.
Bioaugmentation, a strategy based on microbiome engineering, has been proposed for bioremediation of pollutant-contaminated environments. However, the complex microbiome engineering processes for soil bioaugmentation, involving interactions among the exogenous inoculum, soil environment, and indigenous microbial microbiome, remain largely unknown. Acetamiprid is a widely used neonicotinoid insecticide which has caused environmental contaminations. Here, we used an acetamiprid-degrading strain, Pigmentiphaga sp. D-2, as inoculum to investigate the effects of bioaugmentation on the soil microbial community and the process of microbiome reassembly. The bioaugmentation treatment removed 94.8 and 92.5% of acetamiprid within 40 days from soils contaminated with 50 and 200 mg/kg acetamiprid, respectively. A decrease in bacterial richness and diversity was detected in bioaugmentation treatments, which later recovered with the removal of acetamiprid from soil. Moreover, the bioaugmentation treatment significantly influenced the bacterial community structure, whereas application of acetamiprid alone had little influence on the soil microbial community. Furthermore, the bioaugmentation treatment improved the growth of bacteria associated with acetamiprid degradation, and the inoculated and recruited taxa significantly influenced the keystone taxa of the indigenous microbiome, resulting in reassembly of the bacterial community yielding higher acetamiprid-degrading efficiency than that of the indigenous and acetamiprid-treated communities. Our results provide valuable insights into the mechanisms of microbiome engineering for bioaugmentation of acetamiprid-contaminated soils.
生物强化，一种基于微生物组工程的策略，已被提议用于受污染环境的生物修复。然而，土壤生物强化的复杂微生物组工程过程，涉及外生接种物、土壤环境和土著微生物微生物组之间的相互作用，在很大程度上仍然未知。乙酰甲胺磷是一种广泛使用的新烟碱类杀虫剂，已造成环境污染。在这里，我们使用一种可降解乙酰甲胺磷的菌株 Pigmentiphaga sp. D-2 作为接种物，研究了生物强化对土壤微生物群落和微生物组再组装过程的影响。生物强化处理在 40 天内分别从含有 50 和 200mg/kg 乙酰甲胺磷的土壤中去除了 94.8%和 92.5%的乙酰甲胺磷。在生物强化处理中，细菌丰富度和多样性降低，随后随着土壤中乙酰甲胺磷的去除而恢复。此外，生物强化处理显著影响了细菌群落结构，而单独施用乙酰甲胺磷对土壤微生物群落几乎没有影响。此外，生物强化处理促进了与乙酰甲胺磷降解相关的细菌的生长，接种和招募的分类群显著影响了土著微生物组的关键类群，导致细菌群落的重新组装产生比土著和乙酰甲胺磷处理群落更高的乙酰甲胺磷降解效率。我们的研究结果为乙酰甲胺磷污染土壤的生物强化微生物组工程机制提供了有价值的见解。
