Department of Endodontics, Faculty of Dentistry, Akdeniz University, Antalya, Turkey.
Department of Endodontics, Faculty of Dentistry, Alanya Alaaddin Keykubat University, Turkey.
Dent Med Probl. 2020 Oct-Dec;57(4):393-400. doi: 10.17219/dmp/123615.
Root canal disinfection includes mechanical, chemical and biological struggle against microorganisms (MOs). Photodynamic therapy (PDT) and nanoparticle (NP) agents may be proposed as an alternative for use against intracanal infections due to their ability to disrupt biofilm and prevent bacterial adhesion to dentin. The use of NP agents in combination with light/photosensitizer (PS) agents increases the efficiency of PDT in root canal disinfection.
The aim of the study was to evaluate the effect of light application - PDT - on the antibacterial activity of the combination of a PS agent (toluidine blue O -TBO) and an NP agent (silver nanoparticles - AgNPs) for the disinfection of the root canals inoculated with Enterococcus faecalis (E. faecalis).
In this study, concentrations of 20 ppm of TBO and 10 ppm of AgNPs, which showed the highest antibacterial activity against E. faecalis in the TBO/AgNPs combination, were used according to the preliminary studies. After instrumentation, 120 human, single-rooted, straight-canal mandibular premolars of a standard length of 13 mm were contaminated with bacteria, and experimental procedures were conducted against 21-day-old mature biofilm. The teeth were randomly divided into 5 main experimental groups: TBO/light; AgNPs; TBO/AgNPs; AgNPs/light; and TBO/AgNPs/light. Then, these main groups were divided into 2 subgroups each, according to the 2 application time periods (30 s and 60 s) (n = 10). The remaining 20 teeth constituted positive and negative control groups. The data was analyzed with the Kolmogorov-Smirnov test, one-way analysis of variance (ANOVA), Tukey's honestly significant difference (HSD) test, and the Bonferroni correction.
The NaOCl group provided a bacterial reduction that was higher than in all other groups in a statistically significant manner. Light application on the TBO/AgNPs combination was the group that provided the highest bacterial reduction after NaOCl.
The photoactivation of the TBO/AgNPs combination led to an increase in the effect of PDT, and it has the potential to be used as an adjunct for disinfection of the root canal system.
根管消毒包括针对微生物（MOs）的机械、化学和生物斗争。光动力疗法（PDT）和纳米颗粒（NP）剂由于能够破坏生物膜并防止细菌黏附到牙本质上，因此可能被提议作为针对根管内感染的替代方法。NP 剂与光/光敏剂（PS）剂联合使用可提高根管消毒中 PDT 的效率。
本研究的目的是评估光应用 - PDT - 对组合 PS 剂（甲苯胺蓝 O -TBO）和 NP 剂（银纳米颗粒 - AgNPs）的抗菌活性的影响，以对接种粪肠球菌（E. faecalis）的根管进行消毒。
在这项研究中，根据初步研究，使用了 TBO/AgNPs 组合中对 E. faecalis 表现出最高抗菌活性的 20ppmTBO 和 10ppmAgNPs 浓度。经过器械处理后，120 个人的、单根、直根管下颌前磨牙，标准长度为 13mm，被细菌污染，并对 21 天龄成熟生物膜进行了实验处理。牙齿被随机分为 5 个主要实验组：TBO/光；AgNPs；TBO/AgNPs；AgNPs/光；TBO/AgNPs/光。然后，根据 2 种应用时间（30 秒和 60 秒）（n=10）将这些主要组分为每个 2 个子组。其余 20 颗牙齿构成阳性和阴性对照组。使用 Kolmogorov-Smirnov 检验、单向方差分析（ANOVA）、Tukey 的诚实显着差异（HSD）检验和 Bonferroni 校正对数据进行分析。
NaOCl 组提供的细菌减少量明显高于所有其他组。在 NaOCl 之后，光应用于 TBO/AgNPs 组合提供了最高的细菌减少量。
TBO/AgNPs 组合的光激活导致 PDT 效果增加，并且有可能被用作根管系统消毒的辅助手段。
