Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Hemmat Highway, Next to Milad Tower, Tehran, Iran.
Molecular and Medicine Research Center, Khomein University of Medical Sciences, Khomein, Iran.
BMC Res Notes. 2022 Feb 24;15(1):84. doi: 10.1186/s13104-022-05973-w.
Streptococcus mutans is one of the principal causative agents of dental caries (tooth decay) found in the oral cavity. Therefore, this study investigates whether selenium nanoparticles (SeNPs) enhance the efficacy of photodynamic therapy (PDT) against both planktonic communities and the one-day-old biofilm of S. mutans. In this study, the planktonic and 24-h biofilm of S. mutans have been prepared in 96-cell microplates. These forms were treated by methylene blue (MB) and SeNPs and then were exposed to light-emitting diode (LED) lighting. Finally, the results have been reported as CFU/ml.
The outcomes demonstrated that MB-induced PDT and SeNPs significantly reduced the number of planktonic bacteria (P-value < 0.001). The comparison between the treated and untreated groups showed that combining therapy with SeNPs and PDT remarkably decreased colony-forming units of one-day-old S. mutans biofilm (P-value < 0.05). The findings revealed that PDT modified by SeNPs had a high potential to destroy S. mutans biofilm. This combination therapy showed promising results to overcome oral infection in dental science.
变形链球菌是口腔中龋齿（蛀牙）的主要病原体之一。因此，本研究旨在探讨硒纳米颗粒（SeNPs）是否能增强光动力疗法（PDT）对浮游群落和一天龄变形链球菌生物膜的疗效。本研究在 96 孔细胞培养板中制备了变形链球菌的浮游和 24 小时生物膜。用亚甲蓝（MB）和 SeNPs 处理这些形态，然后用发光二极管（LED）照射。最后，结果以 CFU/ml 报告。
结果表明，MB 诱导的 PDT 和 SeNPs 显著减少了浮游细菌的数量（P 值<0.001）。处理组与未处理组的比较表明，SeNPs 和 PDT 联合治疗显著降低了一天龄变形链球菌生物膜的菌落形成单位（P 值<0.05）。研究结果表明，经 SeNPs 修饰的 PDT 具有破坏变形链球菌生物膜的高潜力。这种联合治疗方法有望克服口腔医学中的口腔感染。
