蛋白质 N-糖基化和 O-甘露糖基化是由两种进化上相关的 GT-C 糖基转移酶催化的。
Protein N-glycosylation and O-mannosylation are catalyzed by two evolutionarily related GT-C glycosyltransferases.
机构信息
Department of Biochemistry and Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100083, PR China.
Department of Structural Biology, Van Andel Institute, Grand Rapids, MI 49503, United States.
出版信息
Curr Opin Struct Biol. 2021 Jun;68:66-73. doi: 10.1016/j.sbi.2020.12.009. Epub 2021 Jan 11.
Abstract
The structural folds of glycosyltransferases are categorized into three superfamilies: GT-A, GT-B, and GT-C. Few structures of GT-C fold existed in the Protein Data Bank prior to the recent advent of high-resolution cryo-EM, because the glycosyltransferases are large membrane proteins that are difficult to crystallize. The use of cryo-EM has resulted in the structures of several key GT-C glycosyltransferases. Here we summarize the latest structural features of and mechanistic insights into these membrane enzyme complexes.
摘要
糖基转移酶的结构折叠分为三大超家族
GT-A、GT-B 和 GT-C。在最近高分辨率冷冻电镜出现之前，糖基转移酶结构数据库中很少有 GT-C 折叠结构，因为糖基转移酶是大型膜蛋白，难以结晶。冷冻电镜的应用导致了几种关键 GT-C 糖基转移酶结构的出现。在这里，我们总结了这些膜酶复合物的最新结构特征和机制见解。
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