Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia; Center of Excellence in Biotechnology Research, King Saud University, Riyadh, Saudi Arabia.
Chemosphere. 2021 May;270:129474. doi: 10.1016/j.chemosphere.2020.129474. Epub 2020 Dec 29.
The marine bacterium able to consume DDT as the nutrient source was isolated from sea water which was identified as Paracoccus sp. DDT-21 based on 16 S rDNA gene sequence and Gram negative rod, obligate aerobic, non-motile biochemical characteristics. The isolate can degrade over 80% of the DDT, at a concentration of 50 mg/L in MSM in 72 h. Time and pollutant (DDT) dependent growth studies indicated that the isolate Paracoccus sp., DDT-21 significantly degrade the DDT and tolerates under DDT stress up to 50 mg/L. The DDT degradation capability of the strain Paracoccus sp. DDT-21 was found to be 5 ˃ 10 ˃ 15 ˃ 25 ˃ 50 mg/L DDT. The high concentrations (75 and 100 mg/L) of DDT showed significant decrease in DDT degradation. The optimal DDT degradation (∼90.0%) was observed at 6 g/L of yeast extract, 6% of glucose in pH 7.0 at 35 °C with 72 h of incubation as constant. Furthermore, four metabolites were observed by GC-MS analysis such as, DDE, DDD, DDMU, and DDA. The obtained results indicate that the isolate Paracoccus sp. DDT-21 is a promising candidate for the removal and/or detoxification of DDT in the environment.
从海水中分离到一种能够以 DDT 为营养源的海洋细菌，根据 16S rDNA 基因序列和革兰氏阴性棒状、需氧、非运动的生化特性，将其鉴定为副球菌属 DDT-21。该分离物在 MSM 中，在 50mg/L 的浓度下，72h 内可降解超过 80%的 DDT。时间和污染物（DDT）依赖性生长研究表明，分离株副球菌属 DDT-21 能显著降解 DDT，并能耐受高达 50mg/L 的 DDT 胁迫。该菌株 Paracoccus sp. DDT-21 的 DDT 降解能力为 5>10>15>25>50mg/L DDT。高浓度（75 和 100mg/L）的 DDT 显示出 DDT 降解的显著下降。在 6g/L 酵母提取物、6%葡萄糖、pH7.0、35°C 下孵育 72h 时，观察到最佳的 DDT 降解（约 90.0%）。此外，通过 GC-MS 分析观察到四种代谢物，分别为 DDE、DDD、DDMU 和 DDA。研究结果表明，分离株副球菌属 DDT-21 是一种很有前途的候选物，可用于去除和/或解毒环境中的 DDT。
