Rizqi Hamdan Dwi, Purnomo Adi Setyo, Ulfi Aulia
Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya, 60111, Indonesia.
Heliyon. 2023 Jul 13;9(7):e18216. doi: 10.1016/j.heliyon.2023.e18216. eCollection 2023 Jul.
DDT (1,1,1-trichloro-2,2 bis(4-chlorophenyl) ethane) is a synthetic insecticide that has several negative effects on the environment and humans. Therefore, determining an effective method to reduce DDT may give a beneficial impact. Brown-rot fungus, , is well known to have the ability to degrade DDT, even though it might require long-term remediation. In this study, the effect of the addition of bacteria on the biodegradation of DDT by had been investigated. , and were screened for the bacteria which the volume of bacteria at 1, 3, 5, and 10 mL and the time range of addition of bacteria on days 0, 1, 3, and 5. The addition of , and bacteria into the culture increased DDT biodegradation to approximately 62.02; 74.66; and 75.72%, respectively, in which was only able to degrade DDT by 54.52% for 7 days of incubation. enhanced the degradation process, in which the addition of 10 mL of this bacterium at day 1 possessed the highest value of 92.41% within 7 days of incubation. DDD was detected to be a product metabolite through a dechlorination reaction. This study indicated that mixed cultures of and can be used to degrade DDT.
滴滴涕（1,1,1-三氯-2,2-双（4-氯苯基）乙烷）是一种合成杀虫剂，对环境和人类有多种负面影响。因此，确定一种有效的方法来减少滴滴涕可能会产生有益的影响。褐腐真菌虽然可能需要长期修复，但众所周知具有降解滴滴涕的能力。在本研究中，研究了添加细菌对褐腐真菌降解滴滴涕的影响。筛选了芽孢杆菌属、假单胞菌属和伯克霍尔德菌属，研究了1、3、5和10毫升细菌体积以及在第0、1、3和5天添加细菌的时间范围。向褐腐真菌培养物中添加芽孢杆菌属、假单胞菌属和伯克霍尔德菌属细菌后，滴滴涕的生物降解率分别提高到约62.02%、74.66%和75.72%，而褐腐真菌在培养7天时仅能降解54.52%的滴滴涕。芽孢杆菌属增强了降解过程，在第1天添加10毫升这种细菌在培养7天内降解率最高，达到92.41%。通过脱氯反应检测到滴滴滴是一种产物代谢物。本研究表明，芽孢杆菌属和褐腐真菌的混合培养物可用于降解滴滴涕。
