Bhattacharyya Tanima, Kumar Y Pavan, Dash Jyotirmayee
Department of Organic Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700032, India.
ACS Biomater Sci Eng. 2017 Oct 9;3(10):2358-2365. doi: 10.1021/acsbiomaterials.7b00563. Epub 2017 Sep 28.
We herein report that hydrogels can be prepared from guanosine and boronic acids in the presence of K and Pb ions. These supramolecular hydrogels are formed via G-quartet like self-assembly of guanosine and its boronate esters. The potential of this hydrogel construct in mimicking enzyme-like activity has been demonstrated for the first time. We have observed that the self-assembled structure present in K stabilized hydrogel binds to iron(III)-hemin and shows peroxidase activity, catalyzing oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of HO. Furthermore, the conformation of the G-quartet assemblies in the hydrogel can be altered by varying the stabilizing cations K and Pb. This conformational switching has been used to devise a molecular logic gate for sensing of toxic Pb ions.
我们在此报告，在钾离子和铅离子存在的情况下，鸟苷和硼酸可以制备水凝胶。这些超分子水凝胶是通过鸟苷及其硼酸酯的类似G-四联体的自组装形成的。首次证明了这种水凝胶结构在模拟类酶活性方面的潜力。我们观察到，钾稳定化水凝胶中存在的自组装结构与铁（III）-血红素结合，并表现出过氧化物酶活性，在过氧化氢存在的情况下催化3,3',5,5'-四甲基联苯胺（TMB）的氧化。此外，通过改变稳定阳离子钾和铅，可以改变水凝胶中G-四联体组装体的构象。这种构象转换已被用于设计一种用于检测有毒铅离子的分子逻辑门。
