Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.
Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, 130022, China.
Talanta. 2021 Aug 1;230:122351. doi: 10.1016/j.talanta.2021.122351. Epub 2021 Mar 23.
In this paper, an intensive and glow-type chemiluminescence (CL) hydrogel was prepared by simultaneous incorporation of chemiluminescence reagent (luminol) and catalytic cofactor (hemin) into the scaffold of guanosine-derived hydrogel. The self-assembled hydrogel consisted of K stabilized hemin/G-quartet structures, showing significant enzyme-like activity to HO-mediated oxidation of luminol. After adding HO into the hydrogel, blue light visible to naked eyes would come into being and last for over 8 h. The lasting-time CL emission of hydrogel was achieved due to a mechanism of slow-diffusion-controlled heterogeneous catalysis. Moreover, this self-assembled hydrogel performed a good response to HO and the CL emission images could be recorded by smartphone. The hydrogel could remain excellent lifetime stability for months and the stable, enhanced and glow-type CL emission could improve the reliability and precision of CL detection, which has a promising application in cold light source and HO detection of real biological samples.
本文将化学发光（CL）试剂（鲁米诺）和催化辅因子（血红素）同时掺入到由鸟嘌呤衍生的水凝胶支架中，制备了一种强发光型 CL 水凝胶。自组装水凝胶由 K 稳定的血红素/G-四链体结构组成，对 HO 介导的鲁米诺氧化具有显著的酶样活性。将 HO 添加到水凝胶中后，会产生肉眼可见的蓝光，持续时间超过 8 小时。水凝胶的持续 CL 发射归因于慢扩散控制的非均相催化机制。此外，这种自组装水凝胶对 HO 有很好的响应，并且可以通过智能手机记录 CL 发射图像。水凝胶可以保持数月的优异寿命稳定性，稳定、增强和发光型 CL 发射可以提高 CL 检测的可靠性和精度，在冷光源和实际生物样品中 HO 的检测方面具有广阔的应用前景。
