Wang Yu-Pu, Liao Yu-Te, Liu Chia-Hung, Yu Jiashing, Alamri Hatem R, Alothman Zeid A, Hossain Md Shahriar A, Yamauchi Yusuke, Wu Kevin C-W
Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Department of Urology, Taipei Medical University-Shuang Ho Hospital, No. 291, Jhongjheng Road, Jhonghe Dist., New Taipei City 23561, Taiwan.
ACS Biomater Sci Eng. 2017 Oct 9;3(10):2366-2374. doi: 10.1021/acsbiomaterials.7b00230. Epub 2017 Aug 24.
Chemotherapy of bladder cancer has limited efficacy because of the short retention time of drugs in the bladder during therapy. In this research, nanoparticles (NPs) with a new core/shell/corona nanostructure have been synthesized, consisting of iron oxide (FeO) as the core to providing magnetic properties, drug (doxorubicin) loaded calcium phosphate (CaP) as the shell for pH-responsive release, and arginylglycylaspartic acid (RGD)-containing peptide functionalized alginate as the corona for cell targeting (with the composite denoted as RGD-FeO/CaP/Alg NPs). We have optimized the reaction conditions to obtain RGD-FeO/CaP/Alg NPs with high biocompatibility and suitable particle size, surface functionality, and drug loading/release behavior. The results indicate that the RGD-FeO/CaP/Alg NPs exhibit enhanced chemotherapy efficacy toward T24 bladder cancer cells, owing to successful magnetic guidance, pH-responsive release, and improved cellular uptake, which give these NPs great potential as therapeutic agents for future in vivo drug delivery systems.
膀胱癌化疗疗效有限，原因在于治疗期间药物在膀胱内的保留时间较短。在本研究中，已合成具有新型核/壳/冠层纳米结构的纳米颗粒（NPs），其由作为核心以提供磁性的氧化铁（FeO）、负载药物（阿霉素）的磷酸钙（CaP）作为用于pH响应释放的壳层，以及含精氨酰甘氨酰天冬氨酸（RGD）肽功能化的藻酸盐作为用于细胞靶向的冠层组成（该复合材料记为RGD-FeO/CaP/Alg NPs）。我们优化了反应条件，以获得具有高生物相容性以及合适粒径、表面功能和药物负载/释放行为的RGD-FeO/CaP/Alg NPs。结果表明，RGD-FeO/CaP/Alg NPs对T24膀胱癌细胞表现出增强的化疗疗效，这归因于成功的磁导向、pH响应释放以及改善的细胞摄取，这些特性使这些NPs作为未来体内药物递送系统的治疗剂具有巨大潜力。
