Forget Aurelien, Staehly Camille, Ninan Neethu, Harding Frances J, Vasilev Krasimir, Voelcker Nicolas H, Blencowe Anton
School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia 5000, Australia.
Collaborative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Adelaide, South Australia 5000, Australia.
ACS Biomater Sci Eng. 2017 Oct 9;3(10):2384-2390. doi: 10.1021/acsbiomaterials.7b00297. Epub 2017 Aug 30.
Current organ transplantation protocols require the rapid transport of freshly isolated donor tissue to the recipient patient at the site where the procedure is to be conducted. During transport, the tissue graft can quickly deteriorate as a result of oxygen starvation. In this study, we report the fabrication of oxygen-releasing coatings for improved tissue preservation. The coatings were prepared via the encapsulation of calcium peroxide or urea peroxide microparticles between layers of octadiene plasma polymer films. By varying the thickness of the plasma polymer coating and type of peroxide, formulations were obtained that generate oxygen upon contact with aqueous solutions, while at the same time limiting the amount of toxic reactive oxygen species produced. The optimized coatings were tested under hypoxic conditions using the MIN6 β-cell line, which resulted in a 3-fold increase in the viability of cultured cells. These thin oxygen-releasing coatings can be deposited on a wide range of surfaces, creating a platform for oxygen delivery with the potential to extend the viability of transported tissues and increase the time frame available for graft transport.
当前的器官移植方案要求将新鲜分离的供体组织迅速运送到接受手术的患者所在地点。在运输过程中，组织移植物可能会因缺氧而迅速恶化。在本研究中，我们报告了用于改善组织保存的释氧涂层的制备。这些涂层是通过将过氧化钙或过氧化脲微粒封装在八碳二烯等离子体聚合物薄膜层之间制备的。通过改变等离子体聚合物涂层的厚度和过氧化物的类型，获得了在与水溶液接触时产生氧气的配方，同时限制了有毒活性氧的产生量。使用MIN6 β细胞系在缺氧条件下对优化后的涂层进行了测试，结果使培养细胞的活力提高了3倍。这些薄的释氧涂层可以沉积在各种表面上，创建一个氧气输送平台，有可能延长运输组织的活力，并增加移植物运输的时间范围。
