Rana Karan S, Souza Lucas Pereira de, Isaacs Mark A, Raja Farah N S, Morrell Alexander P, Martin Richard A
Aston Institute of Materials Research, School of Engineering & Applied Science and Aston Research Centre for Healthy Ageing, University of Aston, Birmingham B4 7ET, United Kingdom.
ACS Biomater Sci Eng. 2017 Dec 11;3(12):3425-3432. doi: 10.1021/acsbiomaterials.7b00283. Epub 2017 Oct 11.
In this study, we have developed a series of novel gallium oxide doped bioactive glasses to specifically target osteosarcoma cells while aiding new bone formation. The results show that osteosarcoma (Saos-2) cell death is induced through the addition of gallium oxide. Relative to the gallium-free control glass (0% Ga) glasses containing 1, 2, and 3% Ga decreased Saos-2 cell viability in a dose dependent manner. After 72 h in media preconditioned with 3% Ga Saos-2 cell viability was reduced by over 50%. Corresponding studies undertaken on primary normal human osteoblast cells (NHOst) demonstrated no adverse effects to the gallium containing glasses. Hydroxyapatite formation was observed for all glasses when exposed to simulated body fluid.
在本研究中，我们开发了一系列新型的氧化镓掺杂生物活性玻璃，以特异性靶向骨肉瘤细胞，同时促进新骨形成。结果表明，通过添加氧化镓可诱导骨肉瘤（Saos-2）细胞死亡。相对于不含镓的对照玻璃（0% Ga），含有1%、2%和3% Ga的玻璃以剂量依赖方式降低了Saos-2细胞活力。在用3% Ga预处理的培养基中培养72小时后，Saos-2细胞活力降低了50%以上。对原代正常人成骨细胞（NHOst）进行的相应研究表明，含镓玻璃没有不良影响。当所有玻璃暴露于模拟体液时，均观察到羟基磷灰石形成。
