Stojković Dejan, Drakulić Danijela, Schwirtlich Marija, Rajčević Nemanja, Stevanović Milena, Soković Marina D, Gašić Uroš
Department of Plant Physiology, Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia.
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11042 Belgrade, Serbia.
Pharmaceuticals (Basel). 2021 Jan 12;14(1):55. doi: 10.3390/ph14010055.
(L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of methanolic extract by applying UHPLC-LTQ OrbiTrap MS analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against and species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.
(L.) Hoffm.是一种自古以来就在全球范围内被传统使用的植物。尽管它在不同文化的许多传统药物中被广泛应用，但从科学角度来看，对其研究甚少。胶质母细胞瘤是一种预后较差的肿瘤类型，是成人中最常见且致命的脑肿瘤。目前胶质母细胞瘤的治疗策略包括手术、放疗和化疗。另一方面，已经发现癌症患者由于癌症治疗方法的侵入性，极易受到微生物感染。本研究旨在通过超高效液相色谱-线性离子阱轨道阱质谱分析来研究甲醇提取物的化学特征，并分析其抗胶质母细胞瘤和抗菌活性。本研究表明，甲醇提取物含有酚酸和黄酮类化合物，共鉴定出32种化合物。使用A172胶质母细胞瘤细胞系在体外研究了抗胶质母细胞瘤活性。将提取物对A172细胞的细胞毒性作用与对原代人牙龈成纤维细胞（HGF-1）的相同作用进行了比较。用提取物处理A172细胞后，检测到细胞增殖率降低和细胞形态变化。测试了提取物对[具体细菌和酵母种类未给出]细菌和酵母的抗菌活性。该提取物对测试的细菌和酵母具有活性，可抑制浮游细胞和微生物生物膜。本研究首次详细描述了[植物名称未给出]提取物的化学特征，并对其抗胶质母细胞瘤和抗菌活性进行了科学深入分析。
